The plasma pattern of growth hormone in conscious rats during late pregnancy.
The plasma pattern of growth hormone in conscious rats during late pregnancy.
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妊娠晚期清醒大鼠的血浆生长激素模式。
DOI：
10.1677/joe.0.1240191
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
John
中科院分区：
文献类型：
--
作者：
L. Carlsson;Staffan Edén;John
采用自动重复采血法测定妊娠晚期雌性大鼠血浆GH水平。用脉冲分析计算机程序（PULSAR）分析血浆GH谱。在妊娠第15、18和22天，妊娠雌性动物的平均血浆GH水平约为年龄匹配的非妊娠雌性动物的2倍。基础血浆GH水平也增加，而GH脉冲幅度或频率没有变化。GH释放的增加在妊娠第20天更为明显，与非妊娠雌性相比，平均血浆GH水平增加了4倍。这种增加反映了基础血浆GH水平和GH脉冲幅度的增加，但脉冲频率没有增加。在妊娠第22天分娩的雌性大鼠中，分娩期间基础和平均血浆GH水平升高。妊娠女性在妊娠第18天以45分钟间隔多次静脉输注1微克人GH释放因子类似物（hGRF（1-29）-NH 2）后始终有反应。妊娠大鼠垂体切除后，基础和GRF类似物刺激的血浆GH水平均检测不到。目前的研究表明，在妊娠晚期的基础血浆GH水平的增加和一个显着增加，在基础血浆GH水平和GH脉冲幅度在妊娠第20天。此外，孕鼠垂体切除导致检测不到GH水平，表明妊娠期间高水平的GH来自垂体。
The plasma GH levels of female rats during late pregnancy were determined using an automatic method for repetitive blood sampling from conscious animals. The plasma GH patterns were analysed by a pulse analysis computer program (PULSAR). The mean plasma GH levels were about twofold higher in pregnant females on days 15, 18 and 22 of gestation than in age-matched non-pregnant females. The basal plasma GH levels were also increased, while there was no change in GH pulse amplitude or frequency. The augmentation of GH release was even more pronounced on day 20 of gestation, with a fourfold increase in mean plasma GH levels compared with those in non-pregnant females. This increase reflected an increase in both basal plasma GH levels and GH pulse amplitude, but there was no increase in pulse frequency. In female rats that delivered on day 22 of gestation, the basal and mean plasma GH levels increased during parturition. Pregnant females consistently responded to multiple i.v. infusions of 1 microgram human GH-releasing factor analogue (hGRF(1-29)-NH2) given at 45-min intervals on day 18 of gestation. Both basal and GRF analogue-stimulated plasma GH levels were undetectable after hypophysectomy of pregnant rats. The present study demonstrates an increase in basal plasma GH levels during late pregnancy and a marked increase in both basal plasma GH levels and GH pulse amplitude on day 20 of gestation. Furthermore, hypophysectomy of pregnant rats results in undetectable GH levels, indicating that the high levels of GH during pregnancy are derived from the pituitary.