Mycoviruses related to chrysovirus affect vegetative growth in the rice blast fungus Magnaporthe oryzae
Mycoviruses related to chrysovirus affect vegetative growth in the rice blast fungus Magnaporthe oryzae
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DOI：
10.1099/vir.0.025411-0
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发表时间：
2010-12-01
影响因子：
3.8
通讯作者：
Moriyama, Hiromitsu
中科院分区：
文献类型：
--
作者：
Urayama, Syunichi;Kato, Sachie;Moriyama, Hiromitsu
稻瘟病菌（Magnaporthe oryzae）在稻瘟病菌（M oryzae） S-0412-II 1a分离株中检测到4种分支病毒，分别为dsRNA 1 （3554 bp）、dsRNA 2 （3250 bp）、dsRNA 3 （3074 bp）和dsRNA 4 （3043 bp）。通过分离S-0412-II 1a的单个分生孢子，分离到完全丧失分枝病毒的稻瘟病菌，其菌丝生长和色素沉着正常。含有这些dsRNA的等长病毒颗粒（直径约为35 nm）在CsCl中的浮力密度在1 37 ~ 1 40 g cm（-3）之间。dsRNA 1的单个ORF （3384 nt）编码的基因产物与黄病毒科成员的病毒RNA依赖RNA聚合酶高度同源。值得注意的是，分枝病毒S-0412-II la不仅在宿主细胞中检测到，而且在培养上清中也检测到。将含分枝病毒培养的上清液加入未感染的米霉菌菌丝中，观察到菌丝异常聚集，并从聚集的菌丝中检测到分枝病毒的dsRNA，这种新型的dsRNA分枝病毒被命名为米霉菌黄病毒1号
Mycoviruses causing impaired growth and abnormal pigmentation of the host were found in the rice blast fungus, Magnaporthe oryzae Four dsRNAs, dsRNA 1 (3554 bp), dsRNA 2 (3250 bp), dsRNA 3 (3074 bp) and dsRNA 4 (3043 bp), were detected in isolate S-0412-II 1a of M oryzae By picking up single conidia of S-0412-II 1a, cured strains of the fungus were isolated that had completely lost the mycovirus The cured strains had normal mycelial growth and pigmentation, suggesting that this mycovirus modulates host traits The buoyant densities of isometric virus particles (similar to 35 nm diameter) containing these dsRNAs in CsCl ranged from 1 37 to 1 40 g cm(-3) The single ORF (3384 nt) of dsRNA 1 encoded a gene product highly homologous to the viral RNA-dependent RNA polymerase of members of the family Chrysoviridae It is noteworthy that mycovirus S-0412-II la was detected not only in host cells but also in culture supernatant Furthermore, abnormal aggregation of mycelia was observed after adding the mycovirus-containing culture supernatant to an uninfected strain of M oryzae and mycoviral dsRNAs were detectable from the aggregated mycelia This novel dsRNA mycovirus was named Magnaporthe oryzae chrysovirus 1