Potential repair of free radical adducts of dGMP and dG by a series of reductants. A pulse radiolytic study.
Potential repair of free radical adducts of dGMP and dG by a series of reductants. A pulse radiolytic study.
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DOI：
10.1080/09553008514550101
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
P. O'Neill;P. Chapman
中科院分区：
文献类型：
--
作者：
P. O'Neill;P. Chapman
利用脉冲放射分解技术，已经证明dG和dGMP的羟基自由基加合物与一系列具有不同氧化电位的还原剂在pH 7.0-7.4下通过电子转移过程进行相互作用（k大约1.4-34 X 10(8) dm3 mol-1s-1）。 Br-.2 对 dGMP (dG) 的单电子氧化被证明会导致形成一种物质，其性质与 dGMP 的 OH-自由基加合物的性质相似，具有基于光谱和动力学信息的氧化性质。与 Br-.2 相互作用产生的 dGMP 种类的性质根据 Br-.2 产生特定碱基损伤的能力进行了讨论。这些发现的意义在于羟基自由基损伤的潜在自由基修复和协同效应，其中一种还原剂可以以另一种还原剂为代价来再生。
Using the technique of pulse radiolysis, it has been demonstrated that the interaction of hydroxyl-radical adducts of dG and dGMP with a series of reductants with different oxidation potentials at pH 7.0-7.4 proceeds via an electron transfer process (k approximately 1.4-34 X 10(8) dm3 mol-1s-1). The one-electron oxidation of dGMP (dG) by Br-.2 was shown to result in the formation of a species, the properties of which are similar to those of the OH-radical adduct of dGMP with oxidizing properties based upon both spectral and kinetic information. The nature of the dGMP species produced on interaction with Br-.2 is discussed in terms of the ability of Br-.2 to produce specific base damage. The implications of these findings are presented in terms of potential free radical repair of hydroxyl radical damage and of synergistic effects whereby one reductant may be regenerated at the expense of another reductant.