DNA transport by a micromachined Brownian ratchet device
DNA transport by a micromachined Brownian ratchet device
复制标题
DOI：
10.1073/pnas.96.23.13165
复制
发表时间：
1999-11-09
影响因子：
11.1
通讯作者：
Rothberg, JM
中科院分区：
文献类型：
--
作者：
Bader, JS;Hammond, RW;Rothberg, JM
我们已经用微机械加工了一个硅芯片装置，它用一个布朗棘轮来传输DNA，这个布朗棘轮可以纠正微观粒子的布朗运动。DNA 50聚体的传输特性与理论预测一致，DNA扩散常数与以前的实验结果一致。这种类型的微机器可以为DNA或其他带电物质提供通用泵或分离组件，作为微尺度芯片实验室的一部分。具有减小的特征尺寸的设备可以产生基于尺寸的DNA分子分离，其应用包括检测单核苷酸多态性。
We have micromachined a silicon-chip device that transports DNA with a Brownian ratchet that rectifies the Brownian motion of microscopic particles. Transport properties for a DNA 50-mer agree with theoretical predictions, and the DNA diffusion constant agrees with previous experiments. This type of micromachine could provide a generic pump or separation component for DNA or other charged species as part of a microscale lab-on-a-chip. A device with reduced feature size could produce a size-based separation of DNA molecules, with applications including the detection of single-nucleotide polymorphisms.