Dynamic intracellular distribution of Eaf2 and its potential involvement in UV-Induced DNA damage response.
Dynamic intracellular distribution of Eaf2 and its potential involvement in UV-Induced DNA damage response.
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DOI：
10.1089/dna.2008.0733
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发表时间：
2008-11
影响因子：
3.1
通讯作者：
F. Zhuang;Philip Yen;Jiangyue Zhao;M. Nguyen;M. Jiang;Yi-hsin Liu
中科院分区：
文献类型：
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作者：
F. Zhuang;Philip Yen;Jiangyue Zhao;M. Nguyen;M. Jiang;Yi-hsin Liu
Eaf 2编码一种肿瘤抑制因子，在转录激活、凋亡和胚胎发育中发挥多种功能。在这项研究中，我们利用GFP-EAF 2融合蛋白来描述EAF 2的动态亚细胞运动。GFP-EAF 2优先定位于细胞核，并且在ELL的存在下，其在细胞核斑点中积累。然而，Eaf 2是一种不稳定的核蛋白，其稳定性受血清的影响。此外，我们提供了第一个证据表明Eaf 2的核分布对DNA损伤有反应。紫外线照射后，Eaf 2被重新定位到核仁，这表明Eaf 2可能参与DNA损伤反应。
Eaf2 encodes a tumor suppressor that plays multiple functions in transcriptional activation, apoptosis, and embryonic development. In this study, we utilized GFP-EAF2 fusion protein to describe the dynamic subcellular movement of Eaf2. GFP-EAF2 is preferentially localized to the nucleus, and in the presence of ELL, it accumulates in nuclear speckles. However, Eaf2 is an unstable nuclear protein whose stability is affected by serum. Further, we provided first evidence that nuclear distribution of Eaf2 is responsive to DNA damage. Following UV irradiation, Eaf2 is relocalized to the nucleolus, suggesting a possible functional involvement of Eaf2 in DNA damage response.