Two-phase partition chromatography using soybean oil eliminates pesticide residues in aqueous ginseng extract
Two-phase partition chromatography using soybean oil eliminates pesticide residues in aqueous ginseng extract
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DOI：
10.1016/j.chroma.2004.05.029
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发表时间：
2004-07-09
影响因子：
4.1
通讯作者：
Wee, JJ
中科院分区：
文献类型：
--
作者：
Sohn, SH;Kim, SK;Wee, JJ
本研究旨在开发一种经济、省力、高效的人参提取物中农药残留的消除方法。采用大豆油-蒸馏水或人参水浸膏两相分配法去除农药残留。采用带电子捕获或氮磷检测器的气相色谱仪测定农药含量。在试管中，将代表四类农药(有机氯、有机磷、甲氰菊酯、拟除虫菊酯)的15种农药添加到2毫升豆油中，将豆油与6毫升蒸馏水或10%人参水提取物混合。然后旋转试管(2分钟)，以3000转/分的速度离心15分钟，以分离油层和水层。每一层都被收获并接受农药的定量分析。农药在油水混合物中的平均分配比为94.4+/-6.7%，在油与人参提取物混合物中的平均分配比为105.5+/-6.6%。人参提取物中人参有效成分人参皂苷在油脂处理前后没有明显的定性和定量变化。这些结果表明，大豆油和水相的两相分配层析是一种经济、省力、高效的消除人参提取物中农药残留的可靠方法。(C)2004爱思唯尔B.V.保留所有权利。
This study was carried out to develop a cost-, labor- and efficiency-effective elimination method of pesticide residues in ginseng extract. A two-phase partition method between soybean oil and distilled water or aqueous ginseng extract was employed for the elimination of pesticide residues. Content of the pesticides was determined by gas chromatography with electron capture or nitrogen phosphorus detector. A total of 15 pesticides representing four categories (organochlorine, organophosphorus, carbarnate, pyrethroid) were spiked (ca. 2 ppm) to 2 ml soybean oil in a test tube and the oil was mixed with 6 ml distilled water or 10% aqueous ginseng extract. The test tubes were then vortexed (2 min) and centrifuged at 3000 rpm for 15 min to separate the oil and aqueous layers. Each layer was harvested and subjected to quantitative analysis of pesticides. The average distribution ratio of the pesticides to the oil layer was 94.4 +/- 6.7% in the mixture of the oil and distilled water, and 105.5 +/- 6.6% in the mixture of the oil and ginseng extract. No significant qualitative and quantitative change of ginsenosides, the active ingredients of Panax ginseng, was observed in the ginseng extract before and after the oil treatment. These results suggest that two-phase partition chromatography between soybean oil and the aqueous phase is a cost-, labor- and efficiency-effective reliable method for the elimination of pesticide residues in ginseng extract. (C) 2004 Elsevier B.V. All rights reserved.