Inoculation of several Bursaphelenchus xylophilus group nematodes into adult trees of Pinus thunbergii and their survival in the trees
Inoculation of several Bursaphelenchus xylophilus group nematodes into adult trees of Pinus thunbergii and their survival in the trees
复制标题
DOI：
10.1111/j.1439-0329.2010.00707.x
复制
发表时间：
2011-12-01
期刊：
影响因子：
1.4
通讯作者：
Kanzaki, N.
中科院分区：
文献类型：
--
作者：
Maehara, N.;Aikawa, T.;Kanzaki, N.
为明确伞滑刃线虫对成年松树的致病性，采用6种B. xylophilus group nematodes and ca.以10年生黑松为试材。接种B的无毒分离物（C14-5）的树木。在调查期间，木蠹蛾没有死亡，但与对照相比，木蠹蛾的油树脂分泌量有所下降。接种后15个月，少量B。接种B的树能存活。C14-5。接种了B. mucronatus，短喙牛肝菌B. B. doui，B. luxuriosae，B. B. Conicaudatus和Bursaphelenchus sp.NK224（未描述）的油树脂分泌没有下降，也没有枯萎的外部症状。但接种后9个月，有少量B。luxuriosae在接种线虫的树中存活，尽管没有从接种线虫的树中分离出其它四种线虫。这些结果与我们以前的结果（神崎，N.; Aikawa，T.; Maehara，N.; Ichihara，Y.，2010，J. For. Res.;其中B的无毒分离物（OKD-1）。xylophilus和B. Luxuriosae对3年生幼苗造成水流抑制，但无外部症状。因此，在不能用成年树接种的情况下，用3年生实生苗进行接种试验，可以检验线虫对松树的致病性。
To clarify the pathogenicity of Bursaphelenchus nematodes to adult pine trees, inoculation experiments using six species of B. xylophilus group nematodes and ca. 10-year-old trees of Pinus thunbergii were conducted. Trees inoculated with an avirulent isolate (C14-5) of B. xylophilus did not die during the survey, but showed a decline in oleoresin exudation compared with the controls. Fifteen months after the inoculation, a small number of B. xylophilus survived in a tree inoculated with B. xylophilus C14-5. Trees inoculated with B. mucronatus, B. doui, B. luxuriosae, B. conicaudatus and Bursaphelenchus sp. NK224 (undescribed) showed no decline in oleoresin exudation and no external symptoms of wilt. However, 9 months after the inoculation, a small number of B. luxuriosae survived in a tree inoculated with the nematodes, although four other nematode species were not isolated from trees inoculated with them. These results were approximately consistent with our previous results (Kanzaki, N.; Aikawa, T.; Maehara, N.; Ichihara, Y., 2010, J. For. Res.; in press), in which an avirulent isolate (OKD-1) of B. xylophilus and B. luxuriosae caused water flow inhibition without external symptoms in 3-year-old seedlings. Therefore, to examine the pathogenicity of the nematodes to pines, it is useful to use 3-year-old seedlings in inoculation experiments when adult trees cannot be used.