Synthetic RNAs for Gene Regulation: Design Principles and Computational Tools.
Synthetic RNAs for Gene Regulation: Design Principles and Computational Tools.
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基因调节的合成RNA：设计原理和计算工具。
DOI：
10.3389/fbioe.2014.00065
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发表时间：
2014
影响因子：
5.7
通讯作者：
Croce CM
中科院分区：
文献类型：
--
作者：
Laganà A;Shasha D;Croce CM
使用合成的非编码RNA进行基因表达的转录后调控不仅已成为基因功能研究的标准实验室工具，而且还为设计新的和潜在的有前途的治疗策略开辟了新的前景。生物信息学为研究人员提供了各种工具，用于设计，分析和评估RNAi试剂，如小干扰RNA（siRNA），短发夹RNA（shRNA），人工microRNA（a-miR）和microRNA海绵。最近，一种基于细菌CRISPR-Cas9系统（重复的规则间隔短回文重复序列）的基因组工程新系统被证明具有以更特异的方式在转录和转录后水平调节基因表达的潜力。在这篇简短的综述中，我们介绍了RNAi和CRISPRi设计原理，并讨论了当前设计方法的优点和局限性。
The use of synthetic non-coding RNAs for post-transcriptional regulation of gene expression has not only become a standard laboratory tool for gene functional studies but it has also opened up new perspectives in the design of new and potentially promising therapeutic strategies. Bioinformatics has provided researchers with a variety of tools for the design, the analysis, and the evaluation of RNAi agents such as small-interfering RNA (siRNA), short-hairpin RNA (shRNA), artificial microRNA (a-miR), and microRNA sponges. More recently, a new system for genome engineering based on the bacterial CRISPR-Cas9 system (Clustered Regularly Interspaced Short Palindromic Repeats), was shown to have the potential to also regulate gene expression at both transcriptional and post-transcriptional level in a more specific way. In this mini review, we present RNAi and CRISPRi design principles and discuss the advantages and limitations of the current design approaches.