T mitogens trigger LPS responsiveness in mouse thymus cells.
T mitogens trigger LPS responsiveness in mouse thymus cells.
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T 丝裂原触发小鼠胸腺细胞的 LPS 反应。
DOI：
10.4049/jimmunol.114.3.1004
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发表时间：
1975
影响因子：
4.4
通讯作者：
Richard T. Smith
中科院分区：
文献类型：
--
作者：
James T. Forbes;Y. Nakao;Richard T. Smith
小鼠胸腺细胞基本上对脂多糖（LPS）刺激无反应。然而，当与最低促有丝分裂水平的伴刀豆球蛋白A或亚促有丝分裂比率的丝裂霉素处理的同种异体脾细胞一起培养时，与LPS组合，它们表现出DNA从头合成水平或高于单独由T细胞促分裂原诱导的水平。剂量反应动力学是LPS的特征。含有LPS应答细胞的亚群具有低的净浮力密度。在该模型中，植物血凝素和美洲商陆有丝分裂原均不与LPS协同作用以触发胸腺细胞。这些数据表明，LPS触发可能涉及与T细胞亚群的相互作用。
Mouse thymus cells are essentially unresponsive to lipopolysaccharide (LPS) stimulation. However, when cultured with minimally mitogenic levels of concanavalin A or submitogenic ratios of mitomycin-treated allogeneic spleen cells, in combination with LPS, they demonstrate levels of DNA synthesis de novo or greater than those induced by the T cell mitogen alone. Dose-response kinetics were characteristic of LPS. The subpopulation containing the LPS responsive cells was of low net buoyant density. Neither phytohemagglutinin nor pokeweed mitogen acted synergistically with LPS in this model to trigger thymus cells. The data suggest that LPS triggering may involve interaction with a T cell subpopulation.