Changes in milk fat globule membrane proteome after pasteurization in human, bovine and caprine species
Changes in milk fat globule membrane proteome after pasteurization in human, bovine and caprine species
复制标题
人类、牛和山羊巴氏灭菌后乳脂肪球膜蛋白质组的变化
DOI：
10.1016/j.foodchem.2018.12.015
复制
发表时间：
2019-05-01
期刊：
影响因子：
8.8
通讯作者：
Zhou, Peng
中科院分区：
文献类型：
--
作者：
Ma, Ying;Zhang, Lina;Zhou, Peng
乳脂球膜（MFGM）蛋白已被证明对加工非常敏感。本研究旨在研究巴氏灭菌后人、牛和羊MFGM蛋白的热稳定性。用共聚焦激光扫描显微镜测定乳脂球的大小。利用SDS-PAGE和无标记蛋白质组学技术对MFGM蛋白进行了表征。结果表明，巴氏灭菌对这3个品种的mfg大小分布没有影响。在人、牛和羊的MFGM中分别鉴定出1104、632和137个蛋白。巴氏杀菌后显著改变的蛋白质主要参与脂质合成和分泌以及免疫反应。此外，这些蛋白的变化在物种之间也存在差异，人和羊的MFGM蛋白比牛的MFGM蛋白具有更高的热敏性。综上所述，巴氏灭菌对3个物种的MFGM蛋白组成有不同程度的影响。
Milk fat globule membrane (MFGM) proteins have been shown to be very sensitive to processing. This study aims to investigate the thermos-stability of human, bovine, and caprine MFGM proteins after pasteurization. The milk fat globule size was determined using confocal laser scanning microscopy. The MFGM proteins were characterized using SDS-PAGE and label-free proteomic techniques. The results indicated that pasteurization didn't influence the MFGs size distributions in these three species. A total of 1104, 632, and 137 proteins were identified in human, bovine, and caprine MFGM, respectively. The significantly changed proteins after pasteurization were mainly involved in lipid syntheses and secretion as well as immune response. In addition, the changes of these proteins also differed among species, and a higher thermo-sensitivity was observed in human and caprine MFGM proteins than bovine MFGM proteins. In sum, pasteurization affected MFGM protein composition in different extent among three species.