Biodiesel production in a magnetically fluidized bed reactor using whole-cell biocatalysts immobilized within ferroferric oxide-polyvinyl alcohol composite beads
Biodiesel production in a magnetically fluidized bed reactor using whole-cell biocatalysts immobilized within ferroferric oxide-polyvinyl alcohol composite beads
复制标题
使用固定在四氧化三铁-聚乙烯醇复合珠内的全细胞生物催化剂在磁流化床反应器中生产生物柴油
DOI：
10.1016/j.biortech.2022.127253
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发表时间：
2022-05-05
影响因子：
11.4
通讯作者：
Yao，Jingang
中科院分区：
文献类型：
--
作者：
Liu，Jing;Chen，Guanyi;Yao，Jingang
研究了将枯草芽孢杆菌细胞固定在氧化铁-聚乙烯醇复合微球内构建的磁性全细胞生物催化剂（MWCBs），并利用该催化剂在磁性流化床反应器（MFBR）中将废煎炸油酯交换为生物柴油。考察了生物催化剂浓度、反应物流速、磁场强度和温度等因素对酯交换反应的影响。通过MFBR与mwcb的耦合，实现了生物柴油的连续生产。采用响应面法和Box-Behnken设计对优化条件进行了预测，优化后48 h的生物柴油产率最高可达89.0±0.6%。此外，MFBR中mwcb具有良好的稳定性和可重复利用性，在10次循环后仍保持82.5%以上的生物柴油收率。最后，所得生物柴油的燃料性能达到ASTM和EN标准。本研究表明，MFBR系统中利用mwcb生产生物柴油具有很大的工业化潜力。
Magnetic whole-cell biocatalysts (MWCBs) constructed by immobilizingBacillus subtiliscells within ferroferric oxide-polyvinyl alcohol composite beads were developed and employed to transesterify waste frying oil to biodiesel in a magnetically fluidized bed reactor (MFBR). Effective variables including biocatalysts concentration, reactant flow rate, magnetic field intensity and temperature were evaluated to enhance the transesterification. By coupling MFBR with MWCBs, continuous biodiesel production was achieved. Response surface methodology and Box-Behnken design were employed to predict the optimal conditions and the maximum biodiesel yield reached 89.0 ± 0.6% after 48 h under the optimized conditions. Furthermore, MWCBs displayed satisfactory stability and reusability in MFBR and still maintained a biodiesel yield of more than 82.5% after 10 cycles. Lastly, the fuel properties of the obtained biodiesel met the ASTM and EN standards. The present study revealed that the route of producing biodiesel over MWCBs in the MFBR system showed great potential for industrialization.