Content of phenolics and tannins in leaves and pods of some Acacia and Dichrostachys species and effects on in vitro digestibility
Content of phenolics and tannins in leaves and pods of some Acacia and Dichrostachys species and effects on in vitro digestibility
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一些金合欢属和二穗竹属植物的叶子和豆荚中酚类和单宁的含量及其对体外消化率的影响
DOI：
10.22358/jafs/67758/2003
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发表时间：
2003
影响因子：
1
通讯作者：
T. Fujihara
中科院分区：
文献类型：
--
作者：
C. D. K. Runbanza;M. Shem;R. Otsyina;N. Nishino;T. Ichinohe;T. Fujihara
本文报道了相思属（Acacia）三种植物的四个食荚叶（叶片和荚果）。polyacantha，多刺刺拟步行虫A. toraea，A. nilotica）和Dichrostachys sp.的营养潜力，以确定正常酚类和单宁类抗营养因子（ANF）的水平和对体外饲料消化率（IVD）的影响。总提取酚（TP），单宁（TT）的Folin-Ciocalteu测定，和缩合单宁（CT）的丁醇/盐酸测定估计。采用高效液相色谱法（HPLC）对叶片中的总原花青素进行了分析，将其分解为花青素类黄酮.采用产气法测定了有机物的体外降解率。用聚乙二醇单宁生物测定法评价单宁ANF对IVD的不良影响。粗蛋白质含量在饲料种类和叶片间差异显著（P<0.05）。结果表明，毛穗金穗和多刺金穗金穗的叶片粗蛋白（141-194 g/kg DM）显著高于荚果（133-142 g/kg DM）（P<0.05）;香榧属（Toronto）和重穗木属（Dichrostachys sp.），分别中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）和酸性洗涤木质素（ADL）va* 部分数据已提交给2002年5月21日至24日在韩国济州举行的第四届韩日反刍代谢联合研讨会6通讯作者：电子邮件：fujihara@life.shimane-u.ac.jp 646 BROWSE LEGUMES中的酚和单宁647 RUBANZA CDK饲料种类与叶片间的ET值差异显著（P<0.05）。A.尼罗罗非鱼的NDF、ADF和ADL最低（P<0.05），分别为182、68和44 g/kg DM。A.多刺竹和石竹的NDF和ADF含量最高（P<0.05），分别为416和146 g/kg DM。荚果纤维含量显著高于叶片（P<0.05）。叶片的TP、TT和CT（162、138和55 mg/g DM）分别高于豆荚（112、105和35 mg/g DM）（P<0.05）。原花青素类黄酮（mg/gDM）在0.062（A. nilotica）至5.288（A. toracic），0.188（A. toracum）至4.179（A.多棘棘龙（A. polyacantha）和多棘龙（A. nilotica）和4.392（Dichrostachys sp.）分别用于飞燕草素、矢车菊素和天竺葵素。粗枝叶单宁含量较高，大于5%干物质，在动物饲养和营养中是有益的水平，因此会损害饲料消化率。OMD在饲料种类和叶组分间差异显著（P<0.05）。荚果的产气量和OMD显著高于叶片（P<0.05）。PEG通过结合单宁提高了叶片和荚果的产气量、IVD和代谢能（P<0.05）。叶片对PEG处理的气体产生和IVD的反应均高于荚果，但A.尼罗提卡。改善气体生产和消化率，由于PEG处理表明营养潜力，在浏览饲料以前抑制单宁ANFs。因此，酚类物质和单宁ANF可能会限制利用浏览饲料营养潜力作为补充反刍动物消耗低品质粗饲料。降低单宁抗营养活性可优化粗豆类饲料的利用。在养殖条件下，将产单宁粗饲料与其它饲料混合饲喂，特别是高氮饲料，可降低单宁抗营养活性，达到粗饲料的最佳利用。
Four browse legume foliages (leaves and pod fruits) from three species of Acacia (A. polyacantha, A. tortilis, A. nilotica) and Dichrostachys sp. native to Tanzania were evaluated for nutritive potential to establish levels and effect of normal phenolics and tannins antinutritive factors (ANFs) on in vitro feed digestibility (IVD). Total extractable phenolics (TP), tannins (TT) were estimated by Folin-Ciocalteu assay, and condensed tannins (CT) by butanol/HCl assay. In leaves, total proanthocyanidins were assayed into constituent anthocyanidins’ flavonoids by high performance liquid chromatography (HPLC). Organic matter degradability (OMD) was estimated in vitro by gas production technique. Adverse effects of tannin’s ANF on IVD were assessed by polyethylene glycol (PEG) tannin bioassay. Crude protein (CP) varied (P<0.05) between fodder species and foliages. Leaves had (P<0.05) higher CP (141-194 g/kg DM) in Dichrostachys sp. and A polyacantha, respectively, compared to pods (133-142 g/kg DM) in A. tortilis and Dichrostachys sp., respectively. Neutral detergent fibre (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) va* Part of the data were presented to the 4th Korea-Japan Rumen Metabolism Joint Symposium, May 21st-24th, 2002, Cheju, Republic of Korea 6 Corresponding author: e-mail: fujihara@life.shimane-u.ac.jp 646 PHENOLICS AND TANNINS IN BROWSE LEGUMES 647 RUBANZA C.D.K. ET AL. ried (P<0.05) between fodder species and foliages. A. nilotica had lowest (P<0.05) NDF, ADF and ADL (182, 68 and 44 g/kg DM, respectively). A. polyacantha and Dichrostachys sp. had highest (P<0.05) NDF and ADF (416 and 146 g/kg DM, respectively). Pods had higher (P<0.05) fibre values than leaves. Leaves had higher (P<0.05) TP, TT, and CT (162, 138, and 55 mg/g DM), than pods (112, 105 and 35 mg/g DM), respectively. Proanthocyanidin flavonoids (mg/g DM) varied (P<0.05) from 0.062 (A. nilotica) to 5.288 (A. tortilis), 0.188 (A. tortilis) to 4.179 (A. polyacantha), and 0.009 (A. nilotica) to 4.392 (Dichrostachys sp.) for delphinidins, cyanidins and pelargonidin, respectively. The browse foliages had relatively high tannin content greater than 5% DM, a beneficial level in animal feeding and nutrition, and thus could impair feed digestibility. OMD varied significantly (P<0.05) both between fodder species and foliage components. Pods had higher (P<0.05) gas production (GP) and OMD than leaves. Addition of PEG improved (P<0.05) gas production, IVD and metabolizable energy (ME) in leaves and pods by binding tannins. Leaves had higher response on gas production and IVD due to PEG treatment compared to pods except A. nilotica. Improved gas production and digestibility due to PEG treatment indicate nutritive potential in browse fodder previously depressed by tannin ANFs. Therefore, phenolics and tannins ANFs could limit utilization of browse fodder nutritive potential as supplements to ruminants consuming low quality roughages. Utilization of browse legume fodder could be optimized through reduction of tannin antinutritive activity. Under farmers’ conditions, optimal utilization of browse could be achieved through feeding a mixture of tanniferous browse with other feeds especially high in nitrogen to dilute tannin antinutritive activity.