Effusion and solid lymphomas have distinctive gene and protein expression profiles in an animal model of primary effusion lymphoma
Effusion and solid lymphomas have distinctive gene and protein expression profiles in an animal model of primary effusion lymphoma
复制标题
DOI：
10.1002/path.2012
复制
发表时间：
2006-08
期刊：
影响因子：
--
通讯作者：
Y. Yanagisawa;Y. Sato;Y. Asahi-Ozaki;E. Ito;R. Honma;J. Imai;T. Kanno;M. Kano;H. Akiyama;T. Sata;F. Shinkai-Ouchi;Y. Yamakawa;S. Watanabe;H. Katano
中科院分区：
文献类型：
--
作者：
Y. Yanagisawa;Y. Sato;Y. Asahi-Ozaki;E. Ito;R. Honma;J. Imai;T. Kanno;M. Kano;H. Akiyama;T. Sata;F. Shinkai-Ouchi;Y. Yamakawa;S. Watanabe;H. Katano
淋巴瘤患者通常形成实体瘤，在这些肿瘤中观察到高水平的粘附分子表达。然而，卡波西肉瘤相关疱疹病毒（KSHV）相关原发性积液性淋巴瘤（PEL）不会形成实体瘤，并且细胞中的粘附分子表达受到抑制。将KSHV相关的PEL细胞系接种到严重联合免疫缺陷小鼠的腹腔，导致腹腔内形成积液和实体淋巴瘤。蛋白质组学使用二维凝胶电泳和DNA微阵列分析分别鉴定出14个蛋白和105个基因，其表达在积液性淋巴瘤和实体性淋巴瘤中存在显著差异。五个基因被鉴定出与淋巴细胞功能相关抗原1（白细胞中重要的粘附分子）具有相似的表达谱。其中，冠状蛋白1A是一种肌动蛋白结合蛋白，通过DNA微阵列和蛋白质组学分析被鉴定为在实体淋巴瘤中高表达的分子。Western和northern印迹显示，冠状蛋白1A主要在实体淋巴瘤中表达。此外，与实体性淋巴瘤相比，KSHV编码的溶解蛋白，包括病毒白细胞介素- 6，在积液性淋巴瘤中高表达。这些数据表明，在我们的小鼠模型中，积液性淋巴瘤和实体性淋巴瘤具有不同的基因和蛋白质表达谱，并提示积液性淋巴瘤和实体性淋巴瘤之间基因和蛋白质表达的差异可能与积液性淋巴瘤的形成或实体性淋巴瘤的侵袭性特征有关。此外，结合蛋白质组学和DNA微阵列分析得到的结果表明，蛋白质合成部分反映了mRNA的产生，但与mRNA的产生并不严格相关。版权所有©2006英国和爱尔兰病理学会。约翰·威利父子有限公司出版。
Lymphoma usually forms solid tumours in patients, and high expression levels of adhesion molecules are observed in these tumours. However, Kaposi's sarcoma‐associated herpesvirus (KSHV)‐related primary effusion lymphoma (PEL) does not form solid tumours and adhesion molecule expression is suppressed in the cells. Inoculation of a KSHV‐associated PEL cell line into the peritoneal cavity of severe combined immunodeficiency mice resulted in the formation of effusion and solid lymphomas in the peritoneal cavity. Proteomics using two‐dimensional difference gel electrophoresis and DNA microarray analyses identified 14 proteins and 105 genes, respectively, whose expression differed significantly between effusion and solid lymphomas. Five genes were identified as having similar expression profiles to that of lymphocyte function‐associated antigen 1, an important adhesion molecule in leukocytes. Among these, coronin 1A, an actin‐binding protein, was identified as a molecule showing high expression in solid lymphoma by both DNA microarray and proteomics analyses. Western and northern blotting showed that coronin 1A was predominantly expressed in solid lymphomas. Moreover, KSHV‐encoded lytic proteins, including viral interleukin‐6, were highly expressed in effusion lymphoma compared with solid lymphoma. These data demonstrate that effusion and solid lymphomas possess distinctive gene and protein expression profiles in our mouse model, and suggest that differences in gene and protein expression between effusion and solid lymphomas may be associated with the formation of effusion lymphoma or invasive features of solid lymphoma. Furthermore, the results obtained using this combination of proteomics and DNA microarray analyses indicate that protein synthesis partly reflects, but does not correlate strictly with, mRNA production. Copyright © 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.