fused-somites-like mutants exhibit defects in trunk vessel patterning.
fused-somites-like mutants exhibit defects in trunk vessel patterning.
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融合体节样突变体在干血管模式中表现出缺陷。
DOI：
10.1002/dvdy.20814
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Weinstein,BrantM
中科院分区：
文献类型：
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作者：
Shaw,KennaM;Castranova,DanielA;Pham,VanN;Kamei,Makoto;Kidd,KamehaR;Lo,BrigidD;Torres-Vasquez,Jesus;Ruby,Alexander;Weinstein,BrantM
我们在斑马鱼血管突变体的F3遗传筛查中，在两个不同的基因座上鉴定出四个突变体，显示出类似的躯干血管图案缺陷。在这些突变体中，如果内皮细胞具有适当的规格和分化，最初的血管生成不会受到影响。然而，这四种血管在主干新生血管的生长和构型上都表现出严重的缺陷，节段间血管的异位分支和无定向迁移。这四个突变体在融合体节(FSS)和光束(BEA)基因座上与先前描述的突变体是等位的，并且它们在躯干体节边界的形成方面表现出类似的缺陷。该基因座已被证明与Totbx24相对应；我们在这里证明了斑马鱼lC基因中的束突变是有缺陷的。已知的血管引导因子fNB2a、Sema3a1和Sema3a2的胞体表达是在输入和光束突变体中异常形成的，这表明血管表型是由于这些因子提供的适当指导线索的丢失。发展动力学235：1753-1760,2006。2006年出版，Wiley-Liss，Inc.
We identified four mutants in two distinct loci exhibiting similar trunk vascular patterning defects in an F3 genetic screen for zebrafish vascular mutants. Initial vasculogenesis is not affected in these mutants, with proper specification and differentiation of endothelial cells. However, all four display severe defects in the growth and patterning of angiogenic vessels in the trunk, with ectopic branching and disoriented migration of intersegmental vessels. The four mutants are allelic to previously characterized mutants at thefused‐somites(fss) andbeamter(bea) loci, and they exhibit comparable defects in trunk somite boundary formation. Thefsslocus has been shown to correspond totbx24; we show here thatbeamutants are defective in the zebrafishdlCgene. Somitic expression of known vascular guidance factorsefnb2a,sema3a1, andsema3a2is aberrantly patterned infssandbeamutants, suggesting that the vascular phenotype is due to loss of proper guidance cues provided by these factors. Developmental Dynamics 235:1753–1760, 2006. Published 2006 Wiley‐Liss, Inc.