CD10 expression in extranodal dissemination of Angioimmunoblastic T-cell lymphoma
CD10 expression in extranodal dissemination of Angioimmunoblastic T-cell lymphoma
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DOI：
10.1097/00000478-200401000-00005
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发表时间：
2004-01-01
影响因子：
5.6
通讯作者：
Dogan, A
中科院分区：
文献类型：
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作者：
Attygalle, AD;Diss, TC;Dogan, A
血管免疫母细胞性T细胞淋巴瘤（AITL）是一种全身性疾病，通常有证据表明，在介绍的淋巴结参与。在最近的一项关于AITL淋巴结的研究中，我们发现在大多数情况下肿瘤性T细胞可以通过CD 10的异常表达来识别。本研究的目的是调查是否CD 10表达的肿瘤性T细胞是维持在淋巴结的网站。我们研究了10例具有结外播散的组织学和免疫表型证据的AITL。选择7例未指明的外周T细胞淋巴瘤（PTLu）、2例肠病型T细胞淋巴瘤（ETL）和1例鼻型结内NK/T淋巴瘤作为对照。对诊断性淋巴结活检和淋巴结部位活检进行了回顾。用PCR检测T细胞克隆性，用单层免疫组织化学染色检测CD 3、CD 20、CD 10和CD 21，用双层免疫组织化学染色检测CD 20/CD 10。所有10例ATTL在淋巴结活检中都有特征性的组织学特征和分子证据。在这些病例中，异常的CD 10表达维持在肺、盲肠、扁桃体、鼻咽和六个涉及的骨髓环钻之一中。在这些淋巴结活检中，CD 10阳性肿瘤细胞的分布与滤泡树突状细胞网（FDC）的分布相关。缺乏异常CD 10表达的5例骨髓环钻缺乏FDC的形态学和免疫组化证据。在这5例病例中，有证据表明其他FDC受累部位存在异常CD 10表达。PTLu、ETTL和鼻型结外NK/T淋巴瘤的瘤细胞均为CD 10阴性。我们的数据表明，异常的CD 10表达是一个有用的表型标志物，用于诊断AITL在大多数涉及的结节部位，除了骨髓，并建议FDC在AITL的发病机制中的可能作用。
Angioimmunoblastic T-cell lymphoma (AITL) is a systemic disease that often has evidence of extranodal involvement at presentation. In a recent study of lymph nodes in AITL, we showed that the neoplastic T cells in most cases can be identified by aberrant expression of CD10. The aim of this study was to investigate whether CD10 expression by the neoplastic T cells is maintained in extranodal sites. Ten cases of AITL with histologic and immunophenotypic evidence of extranodal dissemination were studied. Seven cases of peripheral T-cell lymphoma unspecified (PTLu), that included biopsies of involved extranodal sites, two cases of enteropathy type T-cell lymphoma (ETTL), and one case of extranodal NK/T lymphoma, nasal type were selected as controls. Diagnostic lymph node biopsies and biopsies of extranodal sites were reviewed. PCR for T-cell clonality and single layer immunostaining for CD3, CD20, CD10, and CD21 and double layer inummostaining for CD20/CD10 were performed. All 10 cases of ATTL had characteristic histologic features and molecular evidence of the disease in lymph node biopsies. In these cases, aberrant CD10 expression was maintained in the lung, cecum, tonsil, nasopbarynx, and one of six involved bone marrow trephines. In these extranodal biopsies, the distribution of CD10-positive tumor cells correlated with that of the follicular dendritic cell meshwork (FDC). The five bone marrow trephines that lacked aberrant CD10 expression were devoid of morphologic and immunohistochemical evidence of FDC. In these five cases, there was evidence of aberrant CD10 expression in other involved sites that had FDC. The neoplastic cells in PTLu, ETTL, and extranodal NK/T lymphoma, nasal type were CD10 negative. Our data show that aberrant CD10 expression is a useful phenotypic marker for diagnosis of AITL in most involved extranodal sites, except bone marrow, and suggest a possible role of FDC in the pathogenesis of AITL.