VIRUS-INDUCED DIABETES-MELLITUS .6. GENETICALLY DETERMINED HOST DIFFERENCES IN REPLICATION OF ENCEPHALOMYOCARDITIS VIRUS IN PANCREATIC BETA-CELLS
VIRUS-INDUCED DIABETES-MELLITUS .6. GENETICALLY DETERMINED HOST DIFFERENCES IN REPLICATION OF ENCEPHALOMYOCARDITIS VIRUS IN PANCREATIC BETA-CELLS
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DOI：
10.1084/jem.143.5.1170
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发表时间：
1976-01-01
影响因子：
15.3
通讯作者：
NOTKINS, AL
中科院分区：
文献类型：
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作者：
YOON, JW;NOTKINS, AL
从对脑心肌炎(EMC)病毒诱导的糖尿病敏感和抵抗的小鼠品系中分离出β细胞。体内感染EMC病毒的易感小鼠的β细胞比耐药小鼠的β细胞表现出更高的病毒滴度、更严重的脱颗粒和更低的免疫活性胰岛素浓度。免疫荧光和感染中心分析显示，易感小鼠的胰腺含有的感染细胞至少是耐药小鼠胰腺的10倍。从敏感小鼠制备的体外感染的β细胞培养物也显示出比耐药小鼠的β细胞培养物更高的病毒滴度和更严重的细胞病理变化。与贝塔细胞培养相比，病毒在最初的胚胎和肾脏细胞培养中复制得同样好，这些细胞来自敏感和耐药品系的小鼠。结论：EMC病毒诱导的糖尿病的发生与宿主遗传决定的病毒感染β细胞的能力差异有关。
Beta cells were isolated from strains of mice that were susceptible and resistant to encephalomyocarditis (EMC) viral-induced diabetes mellitus. Beta cells from susceptible mice that were infected in vivo with EMC virus showed higher viral titers, more severe degranulation, and lower concentrations of immunoreactive insulin than beta cells from resistant mice. Immunofluorescence and infectious center assays revealed that pancreas from susceptible mice contained at least 10 times more infected cells than pancreas from resistant mice. Beta cell cultures prepared from susceptible mice and infected in vitro also showed higher viral titers and more severe cytopathologic changes than beta cell cultures from resistant mice. In contrast to beta cell cultures, virus replicated equally well in primary embryo and kidney cell cultures from susceptible and resistant strains of mice. It is concluded that the development of EMC virus-induced diabetes is related to genetically determined host differences in the capacity of the virus to infect beta cells.