Maximization of bioconversion of castor oil into ricinoleic acid by response surface methodology.
Maximization of bioconversion of castor oil into ricinoleic acid by response surface methodology.
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DOI：
10.1016/j.biortech.2008.11.040
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发表时间：
2009-09
影响因子：
11.4
通讯作者：
D. Goswami;R. Sen;J. Basu;S. De
中科院分区：
文献类型：
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作者：
D. Goswami;R. Sen;J. Basu;S. De
在这项研究中，响应面法应用于优化工艺变量，如温度，pH值，酶浓度（mg/g油），和缓冲液浓度（g/g油）的水解蓖麻油使用皱褶假丝酵母脂肪酶。采用24完全因子中心组合设计建立了二次回归模型，并对模型进行了优化，得到最佳条件为：温度40°C，pH 7.72，酶浓度5.28mg/g油，缓冲液浓度1g/g油，6 h转化率为65.5%。这些预测的最佳条件与实验结果一致。本文首次报道了响应面法在蓖麻油水解反应中的应用。rugosa脂肪酶在6 h内转化率较高。
In this study, response surface methodology was applied to optimize process variables like temperature, pH, enzyme concentration (mg/g oil), and buffer concentration (g/g oil) for hydrolysis of castor oil using Candida rugosa lipase. A 24full factorial central composite design was used to develop the quadratic model that was subsequently optimized and the optimal conditions were as follows: temperature 40°C, pH 7.72, enzyme concentration 5.28mg/g oil, buffer concentration 1g/g oil and there was 65.5% conversion in 6h. These predicted optimal conditions agreed well with the experimental results. This is the first report on the application of response surface methodology in castor oil hydrolysis using C. rugosa lipase with higher percentage conversion in 6h.