L-forms of H. pylori.
L-forms of H. pylori.
复制标题
L 型幽门螺杆菌。
DOI：
10.3748/wjg.v9.i3.525
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发表时间：
2003
影响因子：
4.3
通讯作者：
Qing
中科院分区：
文献类型：
--
作者：
Ke;Chao;Yu;Ye Tian;Qing
目标
目的：研究消化性溃疡患者幽门螺杆菌L型感染的发生情况及其与患者细胞免疫功能变化的关系。
方法
取2 2 8例消化性溃疡患者胃窦和胃体活检标本，接种于幽门螺杆菌繁殖型Skirrow选择培养基和幽门螺杆菌L型专用培养基，细菌分离鉴定。采用生物素-链霉亲和素(BSA)法检测患者外周血中CD3(+)、CD4(+)、CD8(+)的百分率，用ELISA法检测IL-2、IL-6、IL-8水平。
结果
(1)幽门螺杆菌L型和营养型感染率分别为50.88%(116/228)和64.91%(148/228)，其中L型和营养型混合感染率为78.38%(116/148)。幽门螺杆菌L型在男性和女性患者中的检出率分别为57.04%(77/135)和41.94%(39/93)，差异有统计学意义(P<；0.05)。14岁组、30岁组、40岁组和50岁组幽门螺杆菌L型感染率分别为31.91%(15/47)、42.86%(24/56)、56.94%(41/72)和67.92%(36/53)，差异有统计学意义(P<；0.01)。(2)幽门螺杆菌阳性患者外周血中CD_3~+、CD_4~+、CD_8~+细胞百分率、CD_4~+/CD_8~+比值及IL-2、IL-6、IL-8水平分别为(52.59±5.44)%、(35.51±5.74)%、(27.77±8.64)%、(1.56±0.51)、(2.66±0.47)mg/L。分别为(108.62+/-5.85)ng/L和(115.79+/-7.18)ng/L，与Hp阴性者比较，CD_3(+)、CD_4(+)及CD_4(+)/CD_8(+)比值下降，而IL-2、IL-6水平升高，差异有统计学意义(P<；0.001--P<；在幽门螺杆菌L型和植物型混合感染患者中，CD_3(+)、CD_4(+)、CD_8(+)、CD_4(+)/CD_8(+)比值及IL-2、IL-6、IL-8水平分别为(51.69+/-5.28)%、(34.75+/-5.89)%、(27.15+/-7.45)%、(1.48+/-0.47)%。(2.16+/-0.38)mg/L、(119.45+/-5.44)ng/L、(123.64+/-6.2 4)ng/L，与单纯幽门螺杆菌繁殖型感染者比较，CD_4(+)、CD_4/CD_8(+)比值及IL-2水平升高，而IL-6、IL-8水平下降，差异有统计学意义(P<；0.001-P<；0.05)。
结论
幽门螺杆菌感染的消化性溃疡患者常发生L变异，多见于男性患者，且与年龄有关。幽门螺杆菌L变异可能是幽门螺杆菌感染后消化性溃疡患者细胞免疫功能紊乱的重要因素。
AIM
To study the occurrence of L-forms of H. pylori infection in patients with peptic ulcers and its association with possible changes of cellular immune function in the patients.
METHODS
Endoscopic biopsy specimens of gastric antrum and gastric corpus were taken from 228 patients with peptic ulcers and inoculated into Skirrow selective medium for H. pylori vegetative forms and special medium for H. pylori L-forms, followed by bacterial isolation and identification. And peripheral venous blood of the patients was taken to detect the percentage of CD3(+), CD4(+) and CD8(+) with biotin-streptavidin (BSA) and the level of IL-2, IL-6 and IL-8 with ELISA.
RESULTS
(1) The detection rates of H. pylori L-forms and vegetative forms in the patients were 50.88 % (116/228) and 64.91 % (148/228) respectively, and the co-infection rate of H. pylori L-forms and vegetative forms was 78.38 % (116/148). To be more exact, the detection rates of H. pylori L-forms in male and female patients were 57.04 % (77/135) and 41.94 % (39/93) respectively, and statistics found significant difference between them (P<0.05). Furthermore, the detection rates of H. pylori L-forms in patients aged 14 years-, 30 years-, 40 years- and 50 years- were 31.91 % (15/47), 42.86 % (24/56), 56.94 % (41/72) and 67.92 % (36/53) respectively, and there was significant difference between them (P<0.01). (2) The percentages of CD3(+), CD4(+), CD8(+), the ratio of CD4(+)/CD8(+), and the level of IL-2, IL-6, IL-8 in H. pylori-positive patients were (52.59+/-5.44) %, (35.51+/-5.74) %, (27.77+/-8.64) %, (1.56+/-0.51), (2.66+/-0.47) mg/L, (108.62+/-5.85) ng/L and (115.79+/-7.18) ng/L respectively, compared with those in H. pylori-negative patients, the percentages of CD3(+), CD4(+) and the ratio of CD4(+)/CD8(+) decreased, but the level of IL-2, IL-6 increased, and the difference was significant (P<0.001-P<0.01). Moreover, the percentages of CD3(+), CD4(+), CD8(+), the ratio of CD4(+)/CD8(+), and the level of IL-2, IL-6, IL-8 in the patients with mixed infection of both H. pylori L-forms and vegetative forms were (51.69+/-5.28) %, (34.75+/-5.89) %, (27.15+/-7.45) %, (1.48+/-0.47), (2.16+/-0.38) mg/L, (119.45+/-5.44) ng/L and (123.64+/-6.24) ng/L respectively, compared with those in patients with simple infection of H. pylori vegetative forms, the percentage of CD4(+), the ratio of CD4(+)/CD8(+) and the level of IL-2 increased, but the level of IL-6 and IL-8 decreased, statistical difference was found between them (P<0.001-P<0.05).
CONCLUSION
L-forms variation often occurs in patients with peptic ulcers who are infected by H. pylori, which is commonly found in male patients and related to ages. The L-forms variation of H. pylori can be an important factor causing disorder of cellular immune function in the patients with peptic ulcers who are infected by H. pylori.