Understanding nature's design for a nanosyringe
Understanding nature's design for a nanosyringe
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DOI：
10.1073/pnas.0400352101
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发表时间：
2004-03-30
影响因子：
11.1
通讯作者：
Klein, ML
中科院分区：
文献类型：
--
作者：
Lopez, CF;Nielsen, SO;Klein, ML
合成和天然肽组装体可以通过形成纳米孔而具有跨生物膜的运输或传导活性。抗菌或合成孔形成所需的膜插入的基本机制知之甚少。我们观察到的被动插入到一个模型膜从分子动力学模拟的脂质辅助机制。在研究中使用的组件，一个通用的纳米管功能化的亲水性终端，协助。通过跨叶脂质翻转穿过膜核心。脂质尾部封闭了一个纯疏水的纳米管。所观察到的疏水-亲水匹配的插入机制要求对合成通道和抗生素的设计具有影响。
Synthetic and natural peptide assemblies can possess transport or conductance activity across biomembranes through the formation of nanopores. The fundamental mechanisms of membrane insertion necessary for antimicrobial or synthetic pore formation are poorly understood. We observe a lipid-assisted mechanism for passive insertion into a model membrane from molecular dynamics simulations. The assembly used in the study, a generic nanotube functionalized with hydrophilic termini, is assisted. in crossing the membrane core by transleaflet lipid flips. Lipid tails occlude a purely hydrophobic nanotube. The observed insertion mechanism requirements for hydrophobic-hydrophilic matching have implications for the design of synthetic channels and antibiotics.