Pituitary folliculo-stellate cells immunostained with S-100 protein antiserum in postnatal, castrated and thyroidectomized rats
Pituitary folliculo-stellate cells immunostained with S-100 protein antiserum in postnatal, castrated and thyroidectomized rats
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用 S-100 蛋白抗血清对出生后、去势和甲状腺切除大鼠的垂体滤泡星状细胞进行免疫染色
DOI：
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发表时间：
2004
影响因子：
3.6
通讯作者：
F. Yoshimura
中科院分区：
文献类型：
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作者：
N. Shirasawa;H. Kihara;S. Yamaguchi;F. Yoshimura
本文对雄性和雌性大鼠一生中的卵泡星状细胞及其相关细胞进行了化学染色研究。用抗S100蛋白血清对远侧部和中间部的边缘层细胞以及滤泡星状细胞进行免疫染色。一张切片中免疫染色的滤泡星状细胞通过其相邻切片的超微结构特性鉴定为自身。促肾上腺皮质激素细胞、促甲状腺激素细胞和催乳素细胞，其特征为星状，不与抗S 100蛋白血清免疫染色。S-100蛋白的反应性出现在出生后第6天的中间部的边缘层细胞，并出现在第10天的远侧部的这些细胞。在第6天之前未出现免疫染色的滤泡星状细胞，但在第10天出现了一些。此后，它们的数量增加，随着年龄的增长，免疫染色更强烈。在去势大鼠中，免疫染色的滤泡星状细胞出现相当多，与周围的促性腺激素细胞的分支胞质突起。然而，在甲状腺切除的大鼠中，滤泡星状细胞缺乏分叉的胞质突起，它们对促甲状腺细胞的地形亲和力可以忽略不计。
SummaryPituitary folliculo-stellate cells and associated cells were studied immunohistochemically throughout the life of male and female rats. The marginal layer cells of the pars distalis and intermedia as well as the folliculo-stellate cells were immunostained with anti-S 100 protein serum. The immunostained folliculo-stellate cells in one section were identified as themselves by their ultrastructural properties on the adjacent section. Corticotrophs, thyrotrophs and prolactin cells, characterized by the stellate shape, were not immunostained with anti-S 100 protein serum. Reactivity for S-100 protein appeared on postnatal Day 6 in the marginal layer cells of the pars intermedia, and appeared on Day 10 in those cells of the pars distalis. No immunostained folliculostellate cells appeared before Day 6, but a few of them appeared on Day 10. Thereafter they increased in number, having more intense immunostaining with advancing age. In castrated rats, the immunostained folliculo-stellate cells appeared quite numerously, with branched cytoplasmic processes surrounding the gonadotrophs. In the thyroidectomized rats, however, folliculo-stellate cells lacked ramified cytoplasmic processes, and their topographic affinity for thyrotrophs was negligible.