Lineage-specific regulators couple cell lineage asymmetry to the transcription of the Caenorhabditis elegans POU gene unc-86 during neurogenesis
Lineage-specific regulators couple cell lineage asymmetry to the transcription of the Caenorhabditis elegans POU gene unc-86 during neurogenesis
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DOI：
10.1101/gad.10.11.1395
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发表时间：
1996-06-01
影响因子：
10.5
通讯作者：
Ruvkun, G
中科院分区：
文献类型：
--
作者：
Baumeister, R;Liu, YX;Ruvkun, G
POU同源框基因unc - 86在秀丽隐杆线虫神经系统发育过程中决定神经母细胞和神经元的特性。在一次不对称的神经母细胞分裂后，unc - 86在27个谱系类别的两个子细胞中的一个中表达，这些谱系类别在功能或位置上没有明显的相关性。我们在此表明，unc - 86转录调控区域可检测细胞谱系的不对称性，以激活两个神经母细胞子细胞中的一个表达unc - 86。不同的调控区域激活特定亚谱系组中的unc - 86表达。因此，unc - 86调控区域整合了不同的细胞谱系不对称信号，以激活多种神经母细胞谱系类别中的unc - 86表达。与这种unc - 86不对称激活的谱系特异性调控相一致，lin - 11（LIM同源框）、ham - 1和lin - 17的突变影响特定细胞谱系中unc - 86表达的不对称性，而lin - 32（achaete/scute家族）、vab - 3（Pax - G同源物）和egl - 5（Abd - B同源物）的突变影响其他细胞谱系中unc - 86表达的建立。unc - 86及其许多调控因子的同源物已被认为参与脊椎动物和无脊椎动物的神经发生控制。这些数据表明，unc - 86在一个系统发育上保守的途径中起作用，该途径将神经母细胞谱系不对称性与多种神经元类型的产生联系起来。
The POU homeo box gene unc-86 specifies neuroblast and neural identities in the developing Caenorhabditis elegans nervous system. After an asymmetric neuroblast division, unc-86 is expressed in one of two daughter cells in 27 lineage classes that are not obviously related by function or position. We show here that unc-86 transcriptional regulatory regions detect cell lineage asymmetry to activate unc-86 expression in one of two neuroblast daughter cells. Distinct regulatory regions activate unc-86 expression in particular sets of sublineages. Therefore the unc-86 regulatory region integrates distinct cell lineage asymmetry cues to activate unc-86 expression in the many classes of neuroblast cell lineages. In agreement with such lineage-specific regulation of unc-86 asymmetric activation, mutations in lin-11 (LIM homeo box), ham-1, and lin-17 affect the asymmetry of unc-86 expression in particular cell lineages, and mutations in lin-32 (achaete/scute family), vab-3 (Pax-G homolog) and egl-5 (Abd-B homolog) affect the establishment of unc-86 expression in other cell lineages. Homologs of unc-86 and many of these unc-86 regulators have been implicated in control of neurogenesis in vertebrates and invertebrates. These data suggest that unc-86 acts in a phylogenetically conserved pathway that couples neuroblast cell lineage asymmetry to the generation of diverse neural types.