Long non-coding RNA FEZF1-AS1 facilitates cell proliferation and migration in colorectal carcinoma.
Long non-coding RNA FEZF1-AS1 facilitates cell proliferation and migration in colorectal carcinoma.
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长非编码RNA FEZF1-AS1促进结直肠癌细胞增殖和迁移
DOI：
10.18632/oncotarget.7168
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发表时间：
2016-03-08
期刊：
影响因子：
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通讯作者：
Zhou J
中科院分区：
文献类型：
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作者：
Chen N;Guo D;Xu Q;Yang M;Wang D;Peng M;Ding Y;Wang S;Zhou J
长非编码RNA(LncRNA)在肿瘤的发生发展中起着重要作用。在这里，我们发现了一种新的长非编码RNA(LncRNA)FEZF1反义RNA1(FEZF1-AS1)在人类原发性结直肠癌(CRC)中显著上调，并与CRC转移和预后不良相关。此外，FEZF1-AS1表达下调显著抑制了结直肠癌细胞的增殖、迁移和侵袭，在体外抑制了S期的进入，在体内抑制了肿瘤的生长和转移。相反，FEZF1-AS1过表达可促进结直肠癌细胞的侵袭行为。我们进一步发现，FEZF1-AS1的下调降低了其正义同源基因FEZF1在大肠癌细胞中的mRNA和蛋白表达。FEZF1-AS1与FEZF1在结直肠癌中的表达呈正相关。此外，FEZF1基因敲除还显著抑制了结直肠癌细胞的增殖、迁移和侵袭。我们的研究结果表明，FEZF1-AS1的失调参与了结直肠癌的发生和发展，这可能至少部分是通过FEZF1的诱导来实现的。
Long non-coding RNAs (lncRNA) have been shown to play important roles in the development and progression of cancer. Here, we discovered a novel long noncoding RNA (lncRNA) FEZF1 antisense RNA1 (FEZF1-AS1) is markedly upregulated in human primary colorectal carcinoma (CRC) and associated with CRC metastasis and poor prognosis. Moreover, the downregulation of FEZF1-AS1 expression significantly inhibited the CRC cells proliferation, migration and invasiveness, suppressed S-phase entry in vitro, and repressed tumor growth and metastasis in vivo. In contrast, overexpression of FEZF1-AS1 could promote the aggressive behaviors of CRC cells. We further discovered that the downregulation of FEZF1-AS1 reduced its sense-cognate gene FEZF1 mRNA and protein expression in CRC cells. There was a positive correlation between FEZF1-AS1 and FEZF1 expression in CRC. Moreover, FEZF1 knockdown also significantly suppressed CRC cell proliferation, migration, and invasion. Our findings indicate that the dysregulation of FEZF1-AS1 participates in colorectal tumorigenesis and progression, which might be achieved, at least in part, through FEZF1 induction.