Analytical methods applied to diverse types of Brazilian propolis.
Analytical methods applied to diverse types of Brazilian propolis.
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DOI：
10.1186/1752-153x-5-27
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发表时间：
2011-06-01
影响因子：
--
通讯作者：
Marcucci MC
中科院分区：
文献类型：
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作者：
Sawaya AC;Barbosa da Silva Cunha I;Marcucci MC
蜂胶是一种蜂产品，主要由植物树脂和蜂蜡组成，因此其化学成分因这些树脂的地理和植物来源以及蜜蜂的种类而异。巴西是世界市场上蜂胶的重要供应国，虽然来自东南部的绿色蜂胶是最为人所知和研究最多的，但也可以发现来自意大利蜜蜂和本地无刺蜂（也称为耳垢）的其他几种蜂胶。蜂胶通常作为提取物食用，因此溶剂类型和所采用的提取程序进一步影响其成分。本文综述了枸杞子的提取方法，枸杞子中树脂、蜡质和不溶物的含量分析，枸杞子中酚类、黄酮类、氨基酸和重金属含量的测定方法。不同的色谱方法适用于分离，鉴定和定量巴西的成分和它们的相对优势进行了讨论，以及直接插入质谱指纹。几个世纪以来，蜂胶一直被用作治疗各种疾病的流行药物。它的抗菌特性，存在于不同来源的真菌中，已被广泛研究。但是，最近，已经评估了不同类型的巴西芦荟的抗寄生虫、抗病毒/免疫刺激、愈合、抗肿瘤、抗炎、抗氧化和镇痛活性。最常用的方法和概述他们的相关结果。
Propolis is a bee product, composed mainly of plant resins and beeswax, therefore its chemical composition varies due to the geographic and plant origins of these resins, as well as the species of bee. Brazil is an important supplier of propolis on the world market and, although green colored propolis from the southeast is the most known and studied, several other types of propolis from Apis mellifera and native stingless bees (also called cerumen) can be found. Propolis is usually consumed as an extract, so the type of solvent and extractive procedures employed further affect its composition. Methods used for the extraction; analysis the percentage of resins, wax and insoluble material in crude propolis; determination of phenolic, flavonoid, amino acid and heavy metal contents are reviewed herein. Different chromatographic methods applied to the separation, identification and quantification of Brazilian propolis components and their relative strengths are discussed; as well as direct insertion mass spectrometry fingerprinting. Propolis has been used as a popular remedy for several centuries for a wide array of ailments. Its antimicrobial properties, present in propolis from different origins, have been extensively studied. But, more recently, anti-parasitic, anti-viral/immune stimulating, healing, anti-tumor, anti-inflammatory, antioxidant and analgesic activities of diverse types of Brazilian propolis have been evaluated. The most common methods employed and overviews of their relative results are presented.