A reliable liquid chromatography-tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods
A reliable liquid chromatography-tandem mass spectrometry method for simultaneous determination of multiple mycotoxins in fresh fish and dried seafoods
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DOI：
10.1016/j.chroma.2015.01.071
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发表时间：
2015-03-27
影响因子：
4.1
通讯作者：
Wu, Aibo
中科院分区：
文献类型：
--
作者：
Sun, Wenshuo;Han, Zheng;Wu, Aibo
建立了一种可靠的液相色谱-串联质谱法（LC-MS/MS）同时测定9种真菌毒素，黄曲霉毒素B1（AFB 1）、黄曲霉毒素B2（AFB 2）、黄曲霉毒素G1（AFG 1）、黄曲霉毒素G2（AFG 2）、赭曲霉毒素A（OTA）、玉米赤霉烯酮（ZEN）、T-2毒素、HT 2毒素和脱氧雪腐镰刀菌烯醇（DON）。特别关注样品前处理，这是一个准确和可靠的分析方法的关键。考虑到基质的高度复杂性，对提取溶剂、时间和温度以及净化柱进行了优化，以提高提取效率并降低基质效应。最佳提取工艺为：乙腈/水/冰醋酸（79/20/1，v/v/v），40 ℃超声提取30 min，正己烷脱脂，Oasis HLB柱净化。通过测定线性（R-2 >= 0.9989）、灵敏度（检出限）等指标，进一步验证了该方法的有效性
A reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of nine mycotoxins, i.e., aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), ochratoxin A (OTA), zearalenone (ZEN), T-2 toxin, HT2 toxin and deoxynivalenol (DON), in fresh fish (muscle and entrails) as well as dried seafoods. Special focus was given to sample pretreatment which is crucial for an accurate and reliable analytical method. With regards to the high complexity of the matrices, extraction solvent, time, and temperature as well as clean-up cartridges were optimized to improve extraction efficiency and reduce matrix effects. The optimum procedure included ultrasound-assisted extraction with acetonitrile/water/acetic acid (79/20/1, v/v/v) at 40 degrees C for 30 min, defatting with n-hexane and purification by Oasis HLB cartridges. The method was further validated by determining the linearity (R-2 >= 0.9989), sensitivity (limit of detection