Effect of salt stress on the accumulation of triterpenoid saponins in aseptic cultured Glycyrrhiza uralensis
Effect of salt stress on the accumulation of triterpenoid saponins in aseptic cultured Glycyrrhiza uralensis
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DOI：
10.1007/s10725-022-00933-7
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发表时间：
2022-11
影响因子：
4.2
通讯作者：
Yuto Nishidono;Kotaro Niwa;Ken-ichiro Tanaka
中科院分区：
文献类型：
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作者：
Yuto Nishidono;Kotaro Niwa;Ken-ichiro Tanaka
甘草（甘草）由甘草属的干燥根和根茎组成，在亚洲和欧洲已被用作药物和甜味剂。由于长期的环境退化和过度采伐已大大减少了甘草的可用天然来源，因此需要开发这种植物的栽培系统。然而，与野生甘草植物相比，大多数栽培甘草植物的次生代谢物积累较低。在这里，我们培养了G。在不同盐度条件下观察乌拉拉菌，并使用液相色谱-质谱法测定盐胁迫对三萜皂苷和ononin（异黄酮）积累的影响。用高达 1.2% NaCl 的处理不会影响 G 的鲜重。乌拉尔塞幼苗根。此外，在 0.3% 和 0.6% NaCl 下培养的样品中，除了叶片数量与对照植物相比有所减少外，没有观察到形态变化。根中甘草酸、甘草皂苷G2、H2和B2的含量随着培养基中盐浓度的增加而增加。相比之下，根部大豆皂苷Bb在1.2%盐浓度下显着下降，其中甘草酸及其类似物的浓度最高。洋葱素含量无显着差异。这些结果表明，盐胁迫优先增强G中甘草甜素和甘草皂苷的产生。乌拉伦西斯。
Glycyrrhizae Radix (licorice) comprises the dried roots and rhizomes of theGlycyrrhizaspecies and has been used as a medicine and sweetener in Asia and Europe. Because prolonged environmental degradation and overharvesting have significantly reduced the available natural sources of Glycyrrhizae Radix, the development of a cultivation system for this plant is needed. However, the accumulation of secondary metabolites is lower in most cultivatedGlycyrrhizaplants compared with that in the wild version. Here, we cultivatedG. uralensisunder various salinity conditions and determined the effect of salt stress on the accumulation of triterpenoid saponins and ononin (isoflavonoid) using liquid chromatography–mass spectrometry. Treatment with up to 1.2% NaCl did not affect the fresh weight of theG. uralensisseedling roots. Moreover, morphological changes were not observed in samples cultivated under 0.3% and 0.6% NaCl, except for a reduction in the number of leaves compared with that of the control plants. The content of glycyrrhizin, licorice-saponins G2, H2, and B2, in the roots increased concomitantly with increasing salt concentration in the medium. In contrast, soyasaponin Bb in the roots decreased significantly at 1.2% salt concentration, in which the concentration of glycyrrhizin and its analogs was the highest. There was no significant difference in the content of ononin. These results demonstrated that salt stress preferentially enhances the production of glycyrrhizin and licorice-saponins inG. uralensis.