Diagnostic utility of cyclin D1 in the diagnosis of small round blue cell tumors in children and adolescents
Diagnostic utility of cyclin D1 in the diagnosis of small round blue cell tumors in children and adolescents
复制标题
DOI：
10.1016/j.humpath.2016.07.038
复制
发表时间：
2017-02-01
期刊：
影响因子：
3.3
通讯作者：
Parenti, Rosalba
中科院分区：
文献类型：
--
作者：
Magro, Gaetano;Salvatorelli, Lucia;Parenti, Rosalba
儿童和青少年的小圆形蓝细胞瘤（SRBCT）通常是诊断上具有挑战性的病变。随着基于小活检的诊断方法的增加，需要特定的免疫标记物来帮助不同肿瘤组织型之间的鉴别诊断，以确保患者得到正确的诊断以进行适当的治疗。基于我们最近的研究显示尤文肉瘤/原始周围神经外胚层肿瘤（EWS/pPNET）和周围神经母细胞肿瘤（神经母细胞瘤和神经节神经母细胞瘤）中细胞周期蛋白 D1 过度表达，我们通过免疫组织化学方法评估了 128 例儿童和青少年 SRBCT 中细胞周期蛋白 D1 的免疫反应性，以评估细胞周期蛋白 D1 的免疫反应性。 确定其在鉴别诊断中的潜在效用。所有 EWS/pPNET 病例以及所有外周神经母细胞肿瘤的未分化/低分化神经母细胞瘤成分均表现出强烈且弥漫的细胞周期蛋白 D1 核染色（> 50% 的肿瘤细胞）。相比之下，横纹肌肉瘤（无论何种亚型）和淋巴母细胞淋巴瘤（B 细胞或 T 细胞前体细胞）中不存在该标志物，而在一些肾母细胞瘤（母细胞成分）和促结缔组织增生性小圆细胞肿瘤病例中，仅局部检测到该标志物（< 5% 的肿瘤细胞）。我们的研究结果表明，细胞周期蛋白 D1 可用作常规标记物的诊断辅助手段，以确认 EWS/pPNET 或神经母细胞瘤/神经节神经母细胞瘤的诊断。它在常规实践中的使用也可能有助于那些应用常规标记物后难以诊断的形态未分化的 SRBCT 病例。 (C) 2016 Elsevier Inc. 保留所有权利。
Small round blue cell tumors (SRBCTs) of children and adolescents are often diagnostically challenging lesions. With the increasing diagnostic approach based on small biopsies, there is the need of specific immunomarkers that can help in the differential diagnosis among the different tumor histotypes to assure the patient a correct diagnosis for proper treatment. Based on our recent studies showing cyclin D1 overexpression in both Ewing sarcoma/primitive peripheral neuroectodermal tumor (EWS/pPNET) and peripheral neuroblastic tumors (neuroblastoma and ganglioneuroblastoma), we immunohistochemically assessed cyclin D1 immunoreactivity in 128 cases of SRBCTs in children and adolescents to establish its potential utility in the differential diagnosis. All cases of EWS/pPNET and the undifferentiated/poorly differentiated neuroblastomatous component of all peripheral neuroblastic tumors exhibited strong and diffuse nuclear staining (> 50% of neoplastic cells) for cyclin D1. In contrast, this marker was absent from rhabdomyosarcoma (regardless of subtype) and lymphoblastic lymphoma, (either B- or T-cell precursors), whereas it was only focally detected (< 5% of neoplastic cells) in some cases of Wilms tumor (blastemal component) and desmoplastic small round cell tumor. Our findings suggest that cyclin Dl can be exploitable as a diagnostic adjunct to conventional markers in confirming the diagnosis of EWS/pPNET or neuroblastoma/ ganglioneuroblastoma. Its use in routine practice may also be helpful for those cases of SRBCT with undifferentiated morphology that are difficult to diagnose after application of the conventional markers. (C) 2016 Elsevier Inc. All rights reserved.