A putative mesenchymal stem cells population isolated from adult human testes
A putative mesenchymal stem cells population isolated from adult human testes
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DOI：
10.1016/j.bbrc.2009.05.103
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发表时间：
2009-08-07
影响因子：
3.1
通讯作者：
Patel, A.
中科院分区：
文献类型：
--
作者：
Gonzalez, R.;Griparic, L.;Patel, A.
据报道，从几种成人组织中分离的间充质干细胞（MSCs）是一种很有前途的再生医学工具。为了扩大治疗应用的工具阵列，我们从成人睾丸中分离出一种新的细胞群，称为性腺干细胞（GSCs）。GSCs表达CD105、CD166、CD73、CD90和STRO-1，缺乏造血标志物CD34、CD45和HLA-DR，这些造血标志物是MSCs的特征标识符。此外，GSCs表达多能性标记物Oct4、Nanog和SSEA-4。GSCs繁殖至少64倍，并表现出克隆生成能力。GSCs具有广泛的可塑性和分化成脂肪细胞、成骨细胞和软骨细胞的潜力。这些研究表明，GSCs是一种容易获得的干细胞，具有与MSCs相似的生长动力学和标志物表达，并能分化为中胚层谱系细胞。因此，GSCs可能是一种有价值的治疗应用工具。(c) 2009爱思唯尔公司版权所有。
Mesenchymal stem cells (MSCs) isolated from several adult human tissues are reported to be a promising tool for regenerative medicine. In order to broaden the array of tools for therapeutic application, we isolated a new population of cells from adult human testis termed gonadal stem cells (GSCs). GSCs express CD105, CD166, CD73, CD90, STRO-1 and lack hematopoietic markers CD34, CD45, and HLA-DR which are characteristic identifiers of MSCs. In addition, GSCs express pluripotent markers Oct4, Nanog, and SSEA-4. GSCs propagated for at least 64 population doublings and exhibited clonogenic capability. GSCs have a broad plasticity and the potential to differentiate into adipogenic, osteogenic, and chondrogenic cells. These Studies demonstrate that GSCs are easily obtainable stem cells, have growth kinetics and marker expression similar to MSCs, and differentiate into mesodermal lineage cells. Therefore, GSCs may be a valuable tool for therapeutic applications. (c) 2009 Elsevier Inc. All rights reserved.