Influence of the degree of acetylation on some biological properties of chitosan films
Influence of the degree of acetylation on some biological properties of chitosan films
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DOI：
10.1016/s0142-9612(00)00183-6
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发表时间：
2001-02-01
期刊：
影响因子：
14
通讯作者：
Domard, A
中科院分区：
文献类型：
--
作者：
Chatelet, C;Damour, O;Domard, A
在这项研究中，我们在体外的乙酰化程度（DA）的壳聚糖膜的一些生物学性能的作用进行了研究。我们注意到，无论DA如何，所有壳聚糖膜都对角质形成细胞和成纤维细胞具有细胞相容性。我们还表明，较高的DA的壳聚糖，较低的是细胞粘附在膜上。在这些材料上，成纤维细胞的粘附力似乎是角质形成细胞的两倍。我们观察到，角质形成细胞增殖增加时，DA的壳聚糖膜降低。因此，DA以与细胞粘附相同的方式影响细胞生长。另一方面，尽管成纤维细胞仍然存活，但它们在壳聚糖膜上不增殖。这种行为与这种材料的极高粘附性有关，这肯定会抑制细胞生长。总之，DA在细胞粘附和增殖中起关键作用，但不改变壳聚糖的细胞相容性。同时，注意材料表面形态所起的作用也很重要，这是影响粘附机制的第二个主要参数。(C)2000爱思唯尔科技有限公司版权所有。
In this study, we investigated in vitro the role of the degree of acetylation (DA) on some biological properties of chitosan films. We noticed that, whatever the DA, all chitosan films were cytocompatible towards keratinocytes and fibroblasts. We also demonstrated that the higher the DA of chitosan, the lower was the cell adhesion on the films. Fibroblasts appear to adhere twice as much as keratinocytes on these materials. We observed that keratinocyte proliferation increases when the DA of chitosan films decreases. Thus, DA influences the cell growth in the same way as cell adhesion. On the other hand, although they remain alive, fibroblasts do not proliferate on chitosan films. This behaviour is related to an extremely high adhesion on this kind of material, which certainly inhibits cell growth. In conclusion, DA plays a key role in cell adhesion and proliferation, but does not change the cytocompatibility of chitosan. In parallel, it is also important to notice the role played by the surface morphology of the material, a second major parameter which influences the mechanism of adhesion. (C) 2000 Elsevier Science Ltd. All rights reserved.