VISUALIZATION OF ACTIN FIBERS ASSOCIATED WITH CELL-MEMBRANE IN AMEBAS OF DICTYOSTELIUM-DISCOIDEUM
VISUALIZATION OF ACTIN FIBERS ASSOCIATED WITH CELL-MEMBRANE IN AMEBAS OF DICTYOSTELIUM-DISCOIDEUM
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DOI：
10.1073/pnas.72.5.1758
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
SPUDICH, JA
中科院分区：
文献类型：
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作者：
CLARKE, M;SCHATTEN, G;SPUDICH, JA
将盘基鞭毛虫的阿米巴虫附着在涂有多聚赖氨酸的表面，用缓冲液将细胞的上部剪除。扫描和透射电子显微镜显示，裸露膜的细胞质表面覆盖着由含肌动蛋白的细丝组成的纤维。肌动蛋白通过其溶解性质和与肌肉肌球蛋白相互作用的能力来鉴定。
Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin.