FINE STRUCTURE OF URINARY BLADDER OF TOAD, BUFO MARINUS

FINE STRUCTURE OF URINARY BLADDER OF TOAD, BUFO MARINUS
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DOI:
10.1083/jcb.16.1.53
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发表时间:
1963-01-01
影响因子:
7.8
通讯作者:
CHOI, JK
CHOI, JK
中科院分区:
生物学1区
文献类型:
--
作者:
CHOI, JK

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对蟾蜍膀胱进行了光镜和电镜观察。膀胱壁由上皮、粘膜下层和浆膜组成。在上皮中,四种不同的细胞类型被识别的基础上,他们的精细结构和染色性能与几种不同的染料。这四种细胞分别被命名为颗粒细胞、富含淀粉的细胞、粘液细胞和基底细胞。此外,不同类型的迁移细胞被发现在基底区的上皮。上皮细胞的管腔表面呈现不规则的微绒毛,并被PAS阳性物质包被,其已通过组织化学程序和放射自显影进行了进一步研究。包括细胞膜,细胞连接和细胞内成分的精细结构细节的描述。粘膜下层由成纤维细胞和胶原纤维的精细基质组成,还含有血管和淋巴管、无髓鞘神经、迁移细胞和平滑肌细胞。浆膜由单层浆膜(间皮)细胞组成,形成内脏的不间断覆盖物。讨论了钠和水通过膀胱壁运输的可能途径。
The urinary bladder of the toad (Bufo marinus) was studied with both the light and the electron microscope. The bladder wall consists of epithelium, submucosa, and serosa. In the epithelium, four different cell types were recognized on the basis of their fine structure and staining properties with several different dyes. These four were designated as granular cells, mitochondria-rich cells, mucous cells, and basal cells. In addition, migratory cells of a different type were found in the basal region of the epithelium. The luminal surface of the epithelial cells presents irregular microvilli and is coated by PAS-positive material which has been further investigated by histochemical procedures and radioautography. Included is a description of the fine structural details of cell membranes, cell junctions, and intracellular components. The submucosa consists of a delicate stroma of fibroblasts and collagen fibers and also contains blood and lymph vessels, unmyelinated nerves, migratory cells, and smooth muscle cells. The serosa consists of a single layer of serosal (mesothelial) cells which form an uninterrupted covering of the viscus. Possible pathways of sodium and water transport across the bladder wall are discussed.