YEAST ALPHA-FACTOR IS PROCESSED FROM A LARGER PRECURSOR POLYPEPTIDE - THE ESSENTIAL ROLE OF A MEMBRANE-BOUND DIPEPTIDYL AMINOPEPTIDASE
YEAST ALPHA-FACTOR IS PROCESSED FROM A LARGER PRECURSOR POLYPEPTIDE - THE ESSENTIAL ROLE OF A MEMBRANE-BOUND DIPEPTIDYL AMINOPEPTIDASE
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DOI：
10.1016/0092-8674(83)90070-3
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
THORNER, J
中科院分区：
文献类型：
--
作者：
JULIUS, D;BLAIR, L;THORNER, J
α因子交配信息素是酿酒酵母分泌的一种由13个氨基酸组成的肽。细胞。非交配（不育或不育）。携带STE13基因缺陷的细胞突变体不能产生正常的α。因子，但释放未完全处理的表单集合（.alpha）。因子*)具有显著降低的特定生物活性。主要的。阿尔法。-因子*多肽具有H2N-GluAlaGluAla-.alpha结构。的因素。ste13突变体缺乏一种膜结合的热稳定二肽基氨基肽酶（DPAPase a），该酶特异性地在重复- x - ala -序列的羧基侧进行切割。在遗传杂交中缺乏DPAPase A和其他表型的ste13病变共分离。质粒上克隆的STE13基因导致酵母细胞过量产生DPAPase a数倍。一个不同的克隆DNA片段，微弱地抑制ste13缺陷，导致热不稳定活性（DPAPase B）的过量生产约10倍。其他实验表明，DPAPase A的作用可能对。alpha具有限速作用。-因子成熟正常。α。细胞。
Alpha factor mating pheromone is a peptide of 13 amino acids secreted by S. cerevisiae .alpha. cells. Nonmating (sterile, or ste) .alpha.-cell mutants bearing defects in the STE13 gene do not produce normal .alpha. factor, but release a collection of incompletely processed forms (.alpha. factor*) that have a markedly reduced specific biological activity. The major .alpha.-factor* peptides have the structures H2N-GluAlaGluAla-.alpha. factor. The ste13 mutants lack a membrane-bound heat-stable dipeptidyl aminopeptidase (DPAPase A) that specifically cleaves on the carboxyl side of repeating -X-Ala- sequences. Absence of DPAPase A and the other phenotypes of a ste13 lesion cosegregate in genetic crosses. The cloned STE13 gene on a plasmid causes yeast cells to overproduce DPAPase A severalfold. A different cloned DNA segment, which weakly suppresses the ste13 defects, causes overproduction of a heat-labile activity (DPAPase B) by about 10-fold. Other experiments indicate that DPAPase A action may be rate-limiting for .alpha.-factor maturation in normal .alpha. cells.