Reprogramming the Phenylpropanoid Metabolism in Seeds of Oilseed Rape by Suppressing the Orthologs of REDUCED EPIDERMAL FLUORESCENCE1
Reprogramming the Phenylpropanoid Metabolism in Seeds of Oilseed Rape by Suppressing the Orthologs of REDUCED EPIDERMAL FLUORESCENCE1
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DOI：
10.1104/pp.113.215491
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发表时间：
2013-04-01
期刊：
影响因子：
7.4
通讯作者：
Milkowski, Carsten
中科院分区：
文献类型：
--
作者：
Mittasch, Juliane;Boettcher, Christoph;Milkowski, Carsten
由于类苯丙素途径，许多菊科植物产生大量的可溶性羟基肉桂酸共轭物，主要是芥子酸酯。从油菜（甘蓝型油菜），我们克隆了两个直向同源的拟南芥（拟南芥）基因减少表皮荧光1（REF 1）编码松柏醛/芥子醛脱氢酶。该酶参与从相应的醛形成阿魏酸和芥子酸，从而连接木质素和羟基肉桂酸生物合成作为一个潜在的分支点酶。我们利用RNA干扰技术沉默油菜种子中的REF 1基因。非靶向代谢物分析表明，BnREF 1抑制种子产生了一种新的化学型，其特征在于芥子酸酯水平降低，共轭单木酚类、双木酚类和三木酚类的出现，山奈酚糖苷的积累模式改变，以及咖啡酸、阿魏酸和5-羟基阿魏酸的次要共轭物的变化。BnREF 1抑制影响的水平，次要芥子酸共轭物更严重的主要成分芥子。将改变的代谢物映射到苯丙素代谢网络上，揭示了代谢序列的部分重定向是BnREF 1抑制的主要影响。
As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene REDUCED EPIDERMAL FLUORESCENCE1 (REF1) encoding a coniferaldehyde/sinapaldehyde dehydrogenase. The enzyme is involved in the formation of ferulate and sinapate from the corresponding aldehydes, thereby linking lignin and hydroxycinnamate biosynthesis as a potential branch-point enzyme. We used RNA interference to silence REF1 genes in seeds of oilseed rape. Nontargeted metabolite profiling showed that BnREF1-suppressing seeds produced a novel chemotype characterized by reduced levels of sinapate esters, the appearance of conjugated monolignols, dilignols, and trilignols, altered accumulation patterns of kaempferol glycosides, and changes in minor conjugates of caffeate, ferulate, and 5-hydroxyferulate. BnREF1 suppression affected the level of minor sinapate conjugates more severely than that of the major component sinapine. Mapping of the changed metabolites onto the phenylpropanoid metabolic network revealed partial redirection of metabolic sequences as a major impact of BnREF1 suppression.