Allogeneic offspring produced by male germ line stem cell transplantation into infertile mouse testis
Allogeneic offspring produced by male germ line stem cell transplantation into infertile mouse testis
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DOI：
10.1095/biolreprod.102.008516
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发表时间：
2003-01-01
影响因子：
3.6
通讯作者：
Shinohara, T
中科院分区：
文献类型：
--
作者：
Kanatsu-Shinohara, M;Ogonuki, N;Shinohara, T
睾丸是几个免疫豁免器官之一，以其支持同种异体或异种组织移植的独特能力而闻名。我们研究了在睾丸中移植同种异体雄性生殖系干细胞的小鼠产生后代的可能性。虽然成熟的成年小鼠排斥同种异体生殖细胞，不育，后代获得胞浆内生殖细胞注射使用部分分化的供体细胞。相反，当异基因生殖细胞移植到未成熟的幼鼠睾丸中时，发生了完全的精子发生。通过单克隆抗体给药的耐受性诱导允许幼鼠移植受体通过自然交配产生异基因后代，而未处理受体的附睾中未发现精子。因此，这些结果表明，一个组织不相容的受体可以作为一个“代理父亲”，以传播异源男性供体的遗传信息。
The testis is one of several immune-privileged organs and is known for its unique ability to support allogeneic or xenogeneic tissue transplants. We investigated the possibility of deriving offspring from mice that underwent transplantation with allogeneic male germ line stem cells in the testis. Although mature adult mice rejected allogeneic germ cells and were infertile, offspring were obtained by intracytoplasmic germ cell injection using partially differentiated donor cells. In contrast, complete spermatogenesis occurred when allogeneic germ cells were transplanted into immature pup testes. Tolerance induction by monoclonal antibody administration allowed the pup transplant recipients to produce allogeneic offspring by natural mating, whereas no spermatozoa were found in the epididymis of untreated recipients. Thus, these results, indicate that a histoincompatible recipient can serve as a "surrogate father" to propagate the genetic information of heterologous male donors.