Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids.
Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids.
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DOI：
10.1111/j.1365-2052.1996.tb00489.x
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发表时间：
2009-04
期刊：
影响因子：
2.4
通讯作者：
S. Neuenschwander;G. Rettenberger;E. Meijerink;H. Jörg;G. Stranzinger
中科院分区：
文献类型：
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作者：
S. Neuenschwander;G. Rettenberger;E. Meijerink;H. Jörg;G. Stranzinger
根据人和小鼠肥胖基因（OBS）序列设计简并引物，对猪白色脂肪组织总RNA进行RT-PCR。对所得猪特异性325 bp DNA片段的两条链进行测序。将获得的序列与已知序列进行比较，发现与人的同源性为86%，与小鼠的同源性为84%。利用猪特异性引物，通过体细胞杂种的PCR分析，将OBS基因物理定位到猪18号染色体上。这一结果与最近将人OBS基因分配到7号染色体以及通过比较作图所做的观察一致，即通过使用人7号染色体特异性文库，在猪9号和18号染色体上检测到两个保守同线性区段。我们推断保守同线性的边界在人类染色体的7 q31 - 7 q32区域。
Degenerate primers based on human and mouse obesity gene (OBS) sequencing data were used in the reverse transcriptase-polymerase chain reaction (RT-PCR) of total RNA from pig white adipose tissue. Both strands of the resultant pig- specific 325 bp DNA fragment were sequenced. Comparison of the obtained sequence with known sequences revealed an 86% identity with the human and 84% identity with the mouse OBS cDNA. The OBS gene was physically mapped to pig chromosome 18 by PCR analysis of somatic cell hybrids, using pig-specific primers. This result is consistent with the recent assignment of the human OBS gene to chromosome 7 and the observation made by comparative mapping that by using a human chromosome 7 specific library two segments of conserved synteny were detected on porcine chromosomes 9 and 18. We conclude the border of conserved synteny to be in the 7q31-7q32 region of the human chromosome.