MafT, a new member of the small Maf protein family in zebrafish
MafT, a new member of the small Maf protein family in zebrafish
复制标题
DOI：
10.1016/j.bbrc.2004.05.131
复制
发表时间：
2004-07-16
影响因子：
3.1
通讯作者：
Yamamoto, M
中科院分区：
文献类型：
--
作者：
Takagi, Y;Kobayashi, M;Yamamoto, M
小 Maf 蛋白通过与 CNC 蛋白结合，在形态发生和体内平衡中发挥关键作用。迄今为止，已报道脊椎动物中存在三种小 Maf 蛋白：MafF、MafG 和 MafK，它们具有冗余功能。在这项研究中，我们试图鉴定和表征斑马鱼中的小 Maf 蛋白，以阐明它们在鱼类王国中的保护性和多样性。我们在斑马鱼中鉴定出了 MafG 和 MafK 的同源基因，但没有鉴定出 MafF，这表明前两者在脊椎动物中是保守的。此外，还鉴定出了一种新型的小 Maf 蛋白 MalT。 MafT 蛋白以同二聚体或异二聚体形式与斑马鱼 Nrf2 或 p45 Nfe2 结合 MARE 序列。 MafT 和 Nrf2 的共过表达协同激活斑马鱼胚胎中 MARE 介导的基因表达。这些结果表明MafT是小Maf蛋白的新成员，参与细胞防御系统中Nrf2依赖性基因调控。 (C) 2004 Elsevier Inc. 保留所有权利。
Small Maf proteins play critical roles on morphogenesis and homeostasis through associating with CNC proteins. To date, three small Maf proteins, MafF, MafG, and MafK, have been reported in vertebrates, which share redundant functions. In this study, we tried to identify and characterize small Maf proteins in zebrafish to elucidate their conservation and diversity in the fish kingdom. We identified homolog genes of MafG and MafK but not MafF in zebrafish, indicating the former two are conserved among vertebrates. In addition, a novel type of small Maf protein MalT was identified. MafT protein bound MARE sequence as a homodimer or heterodimers with zebrafish Nrf2 or p45 Nfe2. Co-overexpression of MafT and Nrf2 synergistically activated MARE-mediated gene expression in zebrafish embryos. These results indicated that MafT is a new member of small Maf proteins and involved in the Nrf2-dependent gene regulation in cellular defense system. (C) 2004 Elsevier Inc. All rights reserved.