Protocols for growth and development of Dictyostelium discoideum
Protocols for growth and development of Dictyostelium discoideum
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DOI：
10.1038/nprot.2007.178
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Chisholm, Rex L.
中科院分区：
文献类型：
--
作者：
Fey, Petra;Kowal, Anthony S.;Chisholm, Rex L.
盘基网柄藻是一种能够发育成多细胞结构的单细胞生物，是研究多种生物学过程的重要模式系统。由于它价格便宜且相对容易种植，因此也经常用于教学实验室。在这里，我们描述了成功地生长和发展的Dictyosteoblasts细胞和长期存储的Dictyosteoblasts变形菌和孢子的方法的条件。
Dictyostelium discoideum, a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here we describe conditions for successfully growing and developing Dictyostelium cells and methods for long-term storage of Dictyostelium amoebae and spores.