Genetic analysis of diverse disease-causing pneumococci indicates high levels of diversity within serotypes and capsule switching
Genetic analysis of diverse disease-causing pneumococci indicates high levels of diversity within serotypes and capsule switching
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DOI：
10.1128/jcm.42.12.5681-5688.2004
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发表时间：
2004-12-01
影响因子：
9.4
通讯作者：
Mitchell, TJ
中科院分区：
文献类型：
--
作者：
Jefferies, JMC;Smith, A;Mitchell, TJ
我们已经使用多位点序列分型（MLST）和血清分型，以建立一个系统发育框架的肺炎球菌疾病分离株在苏格兰，提供了一个快照的荚膜类型和基因型之间的关系。结果表明，虽然MLST类型与血清型相关，但血清型内的分离株可以属于许多单独的克隆复合物或序列类型（ST）。我们还表明，相同ST的分离物可以表达不同的荚膜多糖，即，显示囊膜转换，并且对于通常从侵袭性疾病患者分离的囊膜类型和较不常见地从侵袭性疾病患者分离但通常可在人鼻咽中通过病原体携带的血清群都观察到这种现象。
We have used multilocus sequence typing (MLST) and serotyping to build a phylogenetic framework for pneumococcal disease isolates in Scotland that provides a snapshot of the relationships between capsular type and genotype. The results show that while the MLST type correlates with the serotype, isolates within a serotype can belong to a number of individual clonal complexes or sequence types (STs). We also show that isolates of the same ST can express different capsular polysaccharides, i.e., display capsular switching, and that this phenomenon is observed both for capsular types commonly isolated from patients with invasive disease and for serogroups less commonly isolated from patients with invasive disease but which may commonly be carried a symptomatically in the human nasopharynx.