Super-Resolution Optical Fluctuation Bio-Imaging with Dual-Color Carbon Nanodots
Super-Resolution Optical Fluctuation Bio-Imaging with Dual-Color Carbon Nanodots
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DOI：
10.1021/acs.nanolett.5b03609
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发表时间：
2016-01-01
期刊：
影响因子：
10.8
通讯作者：
Chizhik, Alexey I.
中科院分区：
文献类型：
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作者：
Chizhik, Anna M.;Stein, Simon;Chizhik, Alexey I.
超分辨率成像的成功依赖于正确选择荧光探针。在这里，我们提出了一种新的易于生产和生物相容的纳米粒子-碳纳米点-超分辨率光学波动生物成像（SOFT）。这些粒子显示出固有的双色荧光，这对应于不同电荷的粒子的两个亚群。中性纳米粒子定位于细胞核，表明它们作为廉价、容易产生的核特异性标记物的潜在用途。单粒子研究表明，碳纳米点具有独特的荧光特性的混合组合，表现出染料分子和半导体纳米晶体的特性。结果表明，粒子表面的电荷捕获和重新分布触发了它们在发射态和暗态之间的转变。这些发现开辟了新的可能性，利用碳纳米点在各种超分辨率显微镜方法的基础上随机光开关。
Success in super-resolution imaging relies on a proper choice of fluorescent probes. Here, we suggest novel easily produced and biocompatible nanoparticles-carbon nanodots-for super-resolution optical fluctuation bioimaging (SOFT). The particles revealed an intrinsic dual-color fluorescence, which corresponds to two subpopulations of particles of different electric charges. The neutral nanoparticles localize to cellular nuclei suggesting their potential use as an inexpensive, easily produced nucleus-specific label. The single particle study revealed that the carbon nanodots possess a unique hybrid combination of fluorescence properties exhibiting characteristics of both dye molecules and semiconductor nanocrystals. The results suggest that charge trapping and redistribution on the surface of the particles triggers their transitions between emissive and dark states. These findings open up new possibilities for the utilization of carbon nanodots in the various super-resolution microscopy methods based on stochastic optical switching.