Drosulfakinin signaling in fruitless circuitry antagonizes P1 neurons to regulate sexual arousal in Drosophila
Drosulfakinin signaling in fruitless circuitry antagonizes P1 neurons to regulate sexual arousal in Drosophila
复制标题
无果电路中的果磺素信号传导拮抗 P1 神经元来调节果蝇的性唤起
DOI：
10.1038/s41467-019-12758-6
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发表时间：
2019
影响因子：
16.6
通讯作者：
Pan Yufeng
中科院分区：
文献类型：
--
作者：
Wu Shunfan;Guo Chao;Zhao Huan;Sun Mengshi;Chen Jie;Han Caihong;Peng Qionglin;Qiao Huanhuan;Peng Ping;Liu Yan;Luo Shengzhan D;Pan Yufeng
动物通过神经回路整合外部线索和内部状态来执行或终止特定的行为。识别促进或抑制动物行为的神经底物及其分子调节剂是理解神经回路如何控制行为的关键步骤。在这里，我们确定了类胆囊收缩素肽Drosulfakinin（DSK）的功能，在单神经元分辨率，以抑制雄性果蝇的性行为。我们发现Dsk神经元在生理上与男性特异性P1神经元相互作用，P1神经元是男性性行为的指挥中心的一部分，并且相反地调节多种与唤醒相关的行为，包括性，睡眠和自发行走。我们进一步发现DSK-2肽通过其受体CCKLR-17 D3发挥功能，抑制果蝇的性行为。这种神经肽回路与控制男性性行为的大多数方面的无结果表达神经回路在很大程度上重叠。因此，性回路中的DSK/CCKLR信号传导与P1神经元拮抗作用，以平衡唤醒水平并调节性行为。
Animals perform or terminate particular behaviors by integrating external cues and internal states through neural circuits. Identifying neural substrates and their molecular modulators promoting or inhibiting animal behaviors are key steps to understand how neural circuits control behaviors. Here, we identify the Cholecystokinin-like peptide Drosulfakinin (DSK) that functions at single-neuron resolution to suppress male sexual behavior inDrosophila. We found thatDskneurons physiologically interact with male-specific P1 neurons, part of a command center for male sexual behaviors, and function oppositely to regulate multiple arousal-related behaviors including sex, sleep and spontaneous walking. We further found that the DSK-2 peptide functions through its receptor CCKLR-17D3 to suppress sexual behaviors in flies. Such a neuropeptide circuit largely overlaps with thefruitless-expressing neural circuit that governs most aspects of male sexual behaviors. Thus DSK/CCKLR signaling in the sex circuitry functions antagonistically with P1 neurons to balance arousal levels and modulate sexual behaviors.