Cellular content of p53 protein in rat skin after exposure to the space environment.
Cellular content of p53 protein in rat skin after exposure to the space environment.
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暴露于太空环境后大鼠皮肤中 p53 蛋白的细胞含量。
DOI：
10.1152/jappl.1996.81.1.183
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发表时间：
1996
影响因子：
3.3
通讯作者：
Shunji Nagaoka
中科院分区：
文献类型：
--
作者：
Takeo Ohnishi;N. Inoue;H. Matsumoto;T. Omatsu;Y. Ohira;Shunji Nagaoka
空间压力、微重力和空间辐射对生物体的影响仍然未知。我们已经研究了细胞内的p53蛋白质，肿瘤抑制基因的产物，从大鼠皮肤中的蛋白质印迹法使用的印迹扩增系统。使用了三组大鼠。第一组正常地留在地球上，几乎没有检测到任何p53蛋白。第二组在第二次空间实验室生命科学2号使命（F）中进行了为期14天的空间飞行。第三组大鼠除航天外，其余应激与第二组大鼠相同。在返回后第0天（F-0和SC-0组）和第9天（F-9和SC-9组）处死F和SC大鼠。F-0大鼠显示p53蛋白的显著积累，而SC-0大鼠显示轻微下降。F-9和SC-9大鼠显示几乎相同量的p53蛋白，但F-9大鼠显示略高的表达。从这些结果可以看出，暴露于空间环境诱导了大鼠皮肤细胞中细胞p53蛋白的积累。
The effects of stress in space, microgravity and space radiation, on living organisms are still unknown. We have examined the cellular content of p53 protein, a tumor-suppressor gene product, in skin from rats by the Western blot method using a blotting-amplification system. Three groups of rats were used. The first group was kept on Earth normally and showed hardly any detectable p53 protein. The second group made a 14-day flight into space on the second Spacelab Life Sciences-2 mission (F). The last group was subjected to the same kinds of stress as the rats in the second group except for spaceflight (SC). The F and the SC rats were killed on day zero (F-0 and SC-0 groups) and day nine (F-9 and SC-9 groups) after return. F-0 rats showed marked accumulation of p53 protein, whereas SC-0 rats showed a slight decrease. F-9 and SC-9 rats showed almost the same amount of p53 protein, but F-9 rats showed a slightly higher expression. From these results, it is suggested that the accumulation of cellular p53 protein is induced in rat skin cells by exposure to the space environment.