JC virus DNA is present in the mucosa of the human colon and in colorectal cancers
JC virus DNA is present in the mucosa of the human colon and in colorectal cancers
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DOI：
10.1073/pnas.96.13.7484
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发表时间：
1999-06-22
影响因子：
11.1
通讯作者：
Boland, CR
中科院分区：
文献类型：
--
作者：
Laghi, L;Randolph, AE;Boland, CR
JC病毒（JCV）是一种常感染人类的多瘤病毒。我们已经在正常人结肠、结直肠癌、裸鼠结直肠癌异种移植物和人结肠癌细胞系SW 480的粘膜中发现了JCV的T抗原DNA序列。癌细胞中的病毒拷贝数比非肿瘤性结肠细胞中的病毒拷贝数多，并且在单个结肠粘膜标本中存在序列微异质性。用拓扑异构酶I处理后检测的产率提高表明病毒DNA在人体组织中是负超螺旋的。这些结果表明，JCV DNA可以在结肠组织中发现，这提高了这种病毒可能在结直肠癌发生中观察到的染色体不稳定性中发挥作用的可能性。
JC virus (JCV) is a polyoma virus that commonly infects humans. We have found T antigen DNA sequences of JCV in the mucosa of normal human colons, colorectal cancers, colorectal cancer xenografts raised in nude mice, and in the human colon cancer cell line SW480. A larger number of viral copies is present in cancer cells than in non-neoplastic colon cells, and sequence microheterogeneity occurs within individual colonic mucosal specimens. The improved yield of detection after treatment with topoisomerase I suggests that the viral DNA is negatively supercoiled in the human tissues. These results indicate that JCV DNA can be found in colonic tissues, which raises the possibility that this virus may play a role in the chromosomal instability observed in colorectal carcinogenesis.