Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling
Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling
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DOI：
10.1016/j.biomaterials.2009.05.040
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发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Dalby, Matthew J.
中科院分区：
文献类型：
--
作者：
Kantawong, Fahsai;Burgess, Karl E. V.;Dalby, Matthew J.
地形特征可以调节细胞行为，如增殖、迁移、分化和凋亡。生物化学机械转导意味着机械力（例如，来自改变表面形貌的细胞铺展和形态的改变）通过生物分子转化为生物化学信号。尽管如此，很少有人知道哪些途径可能直接参与细胞对材料表面变化的反应。一些途径已经牵连使用集中研究的“选定”的生物分子，而不是一个全球性的信号通路分析。本研究使用受控无序纳米孔形貌（NSQ 50，由电子束光刻制造）来指导祖细胞的成骨细胞分化。这种形貌是独特的，因为它代表了一种中间路线（从绝对有序或随机粗糙度），允许骨转化与地塞米松和抗坏血酸治疗的效率相似。两个直接比较蛋白质组学技术，首先基于凝胶，然后基于色谱法，被用来分析祖细胞蛋白质组的变化，响应于纳米地形。许多改变的蛋白质形成细胞外信号调节激酶（ERK 1/2）途径的一部分。(C)2009爱思唯尔有限公司保留所有权利。
Topographic features can modulate cell behaviours such as proliferation, migration, differentiation and apoptosis. Biochemical mechanotransduction implies the conversion of mechanical forces (e.g. changes in cell spreading and morphology from changing surface topography) into biochemical signal via biomolecules. Still, little is known concerning which pathways may be directly involved in cell response to changes in the material surface. A number of pathways have been implicated using focused studies of 'selected' biomolecules rather than a global analysis of signal pathways. This study used a controlled disorder nanopit topography (NSQ50, fabricated by electron beam lithography) to direct osteoblast differentiation of progenitor cells. This topography is unique as it represents a middle route (from absolute order or random roughness) that allows osteoconversion with similar efficiency as dexamethasone and ascorbate treatment. Two direct-comparison proteomics techniques, firstly gel-based and then chromatography-based, were used to analyse progenitor proteome changes in response to the nanotopography. Many of the changed proteins form part of the Extracellular Signal-regulated Kinase (ERK1/2) pathway. (C) 2009 Elsevier Ltd. All rights reserved.