Folate deficiency decreases apoptosis of endometrium decidual cells in pregnant mice via the mitochondrial pathway.
Folate deficiency decreases apoptosis of endometrium decidual cells in pregnant mice via the mitochondrial pathway.
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作者：
Liao XG;Li YL;Gao RF;Geng YQ;Chen XM;Liu XQ;Ding YB;Mu XY;Wang YX;He JL
众所周知，母体叶酸缺乏会导致不良妊娠结局。除了胚胎发育方面，母体子宫容受性和基质细胞的蜕膜化对于成功怀孕也非常重要。在这项研究中，我们专注于子宫内膜蜕膜化，并调查是否细胞凋亡，这是必不可少的蜕膜化，受损。流式细胞术和TUNEL检测显示，小鼠子宫内膜蜕膜细胞的凋亡抑制饮食叶酸缺乏组在妊娠第7和第8天（第1天=阴道栓）时，蜕膜消退开始。叶酸缺乏组子宫内膜蜕膜组织Bax蛋白表达低于正常饮食组，而Bcl 2蛋白表达与正常饮食组接近。进一步检测发现，正常蜕膜细胞线粒体跨膜电位（Δ Δ Km）降低，激光共聚焦显微镜检测到胞浆弥漫性细胞色素c蛋白的荧光。然而，在叶酸缺乏组中没有观察到相应的变化。蛋白质印迹分析证实，正常蜕膜细胞的线粒体释放出更多的细胞色素c。综上所述，这些结果表明，叶酸缺乏可通过线粒体凋亡途径抑制蜕膜细胞凋亡，从而抑制子宫内膜的蜕膜化，并进一步损害妊娠。
It is well known that maternal folate deficiency results in adverse pregnancy outcomes. In addition to aspects in embryonic development, maternal uterine receptivity and the decidualization of stromal cells is also very important for a successful pregnancy. In this study, we focused on endometrium decidualization and investigated whether apoptosis, which is essential for decidualization, was impaired. Flow cytometry and TUNEL detection revealed that apoptosis of mouse endometrium decidual cells was suppressed in the dietary folate-deficient group on Days 7 and 8 of pregnancy (Day 1 = vaginal plug) when decidua regression is initiated. The endometrium decidual tissue of the folate deficiency group expressed less Bax compared to the normal diet group while they had nearly equal expression of Bcl2 protein. Further examination revealed that the mitochondrial transmembrane potential (ΔΨm) decreased, and the fluorescence of diffuse cytoplasmic cytochrome c protein was detected using laser confocal microscopy in normal decidual cells. However, no corresponding changes were observed in the folate-deficient group. Western blotting analyses confirmed that more cytochrome c was released from mitochondria in normal decidual cells. Taken together, these results demonstrated that folate deficiency could inhibit apoptosis of decidual cells via the mitochondrial apoptosis pathway, thereby restraining decidualization of the endometrium and further impairing pregnancy.
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影响因子：
3.7
作者：
Hsu HC;Chiou JF;Wang YH;Chen CH;Mau SY;Ho CT;Chang PJ;Liu TZ;Chen CH
通讯作者：
Chen CH
影响因子：
3.4
作者：
Bagci, EZ;Vodovotz, Y;Bahar, I
通讯作者：
Bahar, I
DOI：
10.1016/0140-6736(91)90133-a
发表时间：
1991-07-20
期刊：
Lancet (London, England)
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.7
作者：
Novakovic, P;Stempak, JM;Kim, YI
通讯作者：
Kim, YI
影响因子：
3.7
作者：
Ma X;Gao F;Rusie A;Hemingway J;Ostmann AB;Sroga JM;Jegga AG;Das SK
通讯作者：
Das SK