Real-time imaging of the somite segmentation clock: Revelation of unstable oscillators in the individual presomitic mesoderm cells
Real-time imaging of the somite segmentation clock: Revelation of unstable oscillators in the individual presomitic mesoderm cells
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DOI：
10.1073/pnas.0508658103
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发表时间：
2006-01-31
影响因子：
11.1
通讯作者：
Kageyama, R
中科院分区：
文献类型：
--
作者：
Masamizu, Y;Ohtsuka, T;Kageyama, R
Notch信号成分如基本螺旋-环-螺旋基因Hes 1通过负反馈在有丝分裂前中胚层（PSM）中周期性表达，并构成体节分裂时钟。因为Hes 1振荡发生在许多细胞类型中，这个时钟可以调节许多生物系统中的时间。虽然Hes 1振荡器在PSM中是稳定的，但它在其他细胞中迅速衰减，这表明前者和后者中的振荡器可能本质上不同。在这里，我们已经建立了实时生物发光成像系统的Hes 1的表达，并发现，虽然Hes 1振荡是强大的和稳定的PSM，它是不稳定的，在个别解离的PSM细胞，在成纤维细胞。因此，在个人PSM细胞和成纤维细胞的Hes 1振荡器本质上是相似的，这些结果，连同数学模拟，表明，细胞-细胞通信是必不可少的，不仅同步，而且稳定的细胞振荡器。
Notch signaling components such as the basic helix-loop-helix gene Hes1 are cyclically expressed by negative feedback in the presomitic mesoderm (PSM) and constitute the somite segmentation clock. Because Hes1 oscillation occurs in many cell types, this clock may regulate the timing in many biological systems. Although the Hes1 oscillator is stable in the PSM, it damps rapidly in other cells, suggesting that the oscillators in the former and the latter could be intrinsically different. Here, we have established the real-time bioluminescence imaging system of Hes1 expression and found that, although Hes1 oscillation is robust and stable in the PSM, it is unstable in the individual dissociated PSM cells, as in fibroblasts. Thus, the Hes1 oscillators in the individual PSM cells and fibroblasts are intrinsically similar, and these results, together with mathematical simulation, suggest that cell-cell communication is essential not only for synchronization but also for stabilization of cellular oscillators.