Splenectomy exacerbates atrial inflammatory fibrosis and vulnerability to atrial fibrillation induced by pressure overload in rats: Possible role of spleen-derived interleukin-10
Splenectomy exacerbates atrial inflammatory fibrosis and vulnerability to atrial fibrillation induced by pressure overload in rats: Possible role of spleen-derived interleukin-10
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DOI：
10.1016/j.hrthm.2015.07.001
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发表时间：
2016-01-01
期刊：
影响因子：
5.5
通讯作者：
Saikawa, Tetsunori
中科院分区：
文献类型：
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作者：
Kondo, Hidekazu;Takahashi, Naohiko;Saikawa, Tetsunori
背景脾脏在心肌梗死后心脏重构中起重要作用。目的探讨脾切除术（SPX）是否减轻或加重压力超负荷诱导的大鼠心房炎性纤维化和心房颤动（AF）的易感性。6周龄SD大鼠随机分为假手术+假手术组、假手术+SPX组、腹主动脉缩窄术+假手术组和腹主动脉缩窄术+SPX组，分别于术后第2、4、14、28天进行炎症、纤维化和房颤的检测。在脾、血清和左心房（LA）中观察到AAC诱导的白细胞介素-10（IL-10）水平升高，SPX在后两种情况下显示抑制作用。此外，免疫荧光标记显示，SPX可减少AAC诱导的M2巨噬细胞向LA的募集（P
BACKGROUND The spleen is important for cardiac remodeling induced by myocardial infarction. However, the role of the spleen in inflammatory atrial fibrosis induced by pressure overload is unknown.OBJECTIVE The purpose of this study was to investigate whether splenectomy (SPX) attenuates or exacerbates pressure overload-induced atrial inflammatory fibrosis and vulnerability to atrial fibrillation (AF) in rats.METHODS Male Sprague-Dawley rats (6 weeks old) were divided into Sham+Sham, Sham+SPX, abdominal aortic constriction (AAC)+Sham, and AAC+SPX groups, and were evaluated for inflammation, fibrosis, and AF on days 2, 4, 14, and 28.RESULTS On day 4, an AAC-induced rise in interleukin-10 (IL-10) level was observed in the spleen, serum, and left atrium (LA), with SPX showing inhibitory effects in the latter 2 instances. In addition, AAC-induced M2 macrophage recruitment into the LA was decreased by SPX, as determined by immunofluorescence labeling (P