Beneficial effect of enteral glycine in intestinal ischemia/reperfusion injury.
Beneficial effect of enteral glycine in intestinal ischemia/reperfusion injury.
复制标题
肠内甘氨酸对肠道缺血/再灌注损伤的有益作用。
DOI：
10.1007/s11605-006-0011-0
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Daly,JM
中科院分区：
文献类型：
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作者：
Iijima,S;Shou,J;Naama,H;Calvano,SE;Daly,JM
体外研究表明，甘氨酸可保护肾小管和肝细胞免受缺氧损伤。甘氨酸也能减轻移植肝脏的缺血性损伤。本研究探讨了肠内甘氨酸在小肠缺血/再灌注损伤小鼠模型中的作用。将小鼠（每组n = 12）随机接受两次20%甘氨酸（Gly）或23%平衡氨基酸（AA）溶液的灌胃，每次灌胃之间间隔6小时。第二次灌胃后1小时，小鼠进行上级肠系膜动脉夹闭20分钟。然后释放夹钳进行再灌注。另一组小鼠（n = 8）进行假手术，并作为额外的对照动物。缺血/再灌注后6小时，处死小鼠，以评估肠损伤（肠蛋白含量、粘膜二硫代磷酸酶活性和肠组织学检查结果）和全身后果（细菌移位、血清白细胞介素-6和肺髓过氧化物酶活性）。第二组小鼠（n = 55）进行相同的灌胃和缺血/再灌注，并跟踪它们的存活情况。与AA相比，肠缺血/再灌注损伤前给予肠内甘氨酸可显著保护粘膜指数和肠组织学，并降低肺髓过氧化物酶活性。与AA对照小鼠相比，接受甘氨酸的动物的存活率也显著增加。这些数据表明，肠内甘氨酸补充可能是有益的，在减轻肠缺血/再灌注损伤及其相关的全身效应，在这个小鼠模型。
It has been shown in vitro that glycine can protect renal tubules and hepatocytes from hypoxic injury. Glycine also attenuates ischemic injury in transplanted livers. The present study investigated the effect of enteral glycine in a murine model of ischemia/reperfusion injury of the small intestine. Mice (n = 12 in each group) were randomized to receive two gastric gavages of either a 20% glycine (Gly) or 23% balanced amino acid (AA) solution with a 6-hour interval between each gavage. One hour after the second gavage, mice underwent superior mesenteric artery clamping for 20 minutes. The clamp was then released for reperfusion. Another group of mice (n = 8) underwent a sham operation and served as additional control animals. Six hours after ischemia/reperfusion, the mice were killed in order to assess the intestinal injury (intestinal protein content, mucosal disaccharidase activity, and intestinal histologic findings) and the systemic consequences (bacterial translocation, serum interleukin-6, and lung myeloperoxidase activity). A second set of mice (n = 55) underwent identical gavages and ischemia/reperfusion and they were followed for survival. Compared to AA, enteral glycine administered prior to intestinal ischemia/reperfusion injury significantly preserved mucosal indices and intestinal histology and decreased lung myeloperoxidase activity. Survival was also significantly increased in animals receiving glycine compared to AA control mice. These data suggest that enteral glycine supplementation may be beneficial in attenuating intestinal ischemia/reperfusion injury and its related systemic effects in this murine model.