Establishment and early succession of bacterial communities in monochloramine-treated drinking water biofilms
Establishment and early succession of bacterial communities in monochloramine-treated drinking water biofilms
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DOI：
10.1111/1574-6941.12170
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发表时间：
2013-12-01
影响因子：
4.2
通讯作者：
Ashbolt, Nicholas J.
中科院分区：
文献类型：
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作者：
Revetta, Randy P.;Gomez-Alvarez, Vicente;Ashbolt, Nicholas J.
一氯胺是一种越来越多使用的饮用水消毒剂，已被证明会增加饮用水中的硝化细菌和分枝杆菌。在供水系统模拟器中，利用从不同生物膜中产生的16S rRNA基因克隆文库，研究了这些细菌的潜在的继承和发展。使用硼硅酸盐玻璃微珠，以及在一氯胺处理的饮用水中孵化长达8个月的环状反应器中的聚碳酸酯优惠券，从在线和离线设备中获得生物膜。在生物膜装置和优惠券材料之间没有观察到显著的群落结构差异；然而，在不同装置上发展的所有生物膜群落随着时间的推移经历了相似的演替。生物膜形成的早期阶段以沙雷氏菌(29%)、梭状杆菌(23%)、黄杆菌(16%)和假单胞菌(7%)为主，而在随后的几个月中，分枝杆菌样系统型是最主要的种群(27%)。非结核分枝杆菌(NTM)成员在3个月后的发展可能会影响有易感性的个体，而硝化菌(与莫斯科硝化螺旋菌和多形亚硝酸螺旋体相关)可能会影响水质。总体而言，所有克隆文库样本中90%的多样性与变形杆菌门、放线杆菌门和拟杆菌门有关。这些结果为一氯胺处理的饮用水中生物膜细菌的演替提供了一个生态学的见解。
Monochloramine is an increasingly used drinking water disinfectant and has been shown to increase nitrifying bacteria and mycobacteria in drinking waters. The potential successions and development of these bacteria were examined by 16S rRNA gene clone libraries generated from various biofilms within a water distribution system simulator. Biofilms were obtained from in-line and off-line devices using borosilicate glass beads, along with polycarbonate coupons from annular reactors incubated for up to 8months in monochloramine-treated drinking water. No significant difference in community structures was observed between biofilm devices and coupon material; however, all biofilm communities that developed on different devices underwent similar successions over time. Early stages of biofilm formation were dominated by Serratia (29%), Cloacibacterium (23%), Diaphorobacter (16%), and Pseudomonas (7%), while Mycobacterium-like phylotypes were the most predominant populations (>27%) in subsequent months. The development of members of the nontuberculous mycobacteria (NTM) after 3months may impact individuals with predisposing conditions, while nitrifiers (related to Nitrospira moscoviensis and Nitrosospira multiformis) could impact water quality. Overall, 90% of the diversity in all the clone library samples was associated with the phyla Proteobacteria, Actinobacteria, and Bacteroidetes. These results provide an ecological insight into biofilm bacterial successions in monochloramine-treated drinking water.