Role of experimental periodontitis in inducing pulmonary inflammation in mice
Role of experimental periodontitis in inducing pulmonary inflammation in mice
复制标题
实验性牙周炎在诱导小鼠肺部炎症中的作用
DOI：
10.1111/odi.13949
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发表时间：
2021-07-12
期刊：
影响因子：
3.8
通讯作者：
Wang, Zuomin
中科院分区：
文献类型：
--
作者：
Tian, Huan;Zhang, Zheng;Wang, Zuomin
目的 本研究旨在探讨实验性牙周炎在小鼠肺部炎症中的潜在作用。材料和方法将小鼠分为对照组、结扎诱导的牙周炎（L）组和结扎加牙龈卟啉单胞菌（P.gingivalis）诱导的牙周炎（LPG）组。第2、4和8周时检查牙槽骨吸收、肺功能、肺组织组织学和细胞因子表达。然后在8周时进一步评估外周血和肺组织中的细胞因子和中性粒细胞以确定LPG牙周炎诱导的细胞因子的作用，并使用牙龈卟啉单胞菌-IgG和牙龈卟啉单胞菌牙龈蛋白酶评估牙龈卟啉单胞菌的作用。结果L组和LPG组牙槽骨吸收更严重。然而，仅LPG组在第8周时观察到肺部炎症，此时外周血和肺组织中的细胞因子和中性粒细胞升高最显着，同时牙龈卟啉单胞菌-IgG和牙龈卟啉单胞菌牙龈蛋白酶的水平也较高。 L组牙龈组织、外周血和肺组织中细胞因子水平也升高，并伴有外周血中性粒细胞升高，但不如LPG组显着。结论 LPG牙周炎可长期引发肺部炎症，其中细胞因子和牙龈卟啉单胞菌发挥重要作用。
Objective The aim of the study was to explore the potential role of experimental periodontitis in pulmonary inflammation in mice. Materials and methods Mice were divided into control, ligature-induced periodontitis (L) and ligature plus Porphyromonas gingivalis (P. gingivalis)-induced periodontitis (LPG) groups. Alveolar bone resorption, pulmonary function, lung tissue histology and cytokine expression were examined at 2, 4 and 8 weeks. Then cytokines and neutrophils in the peripheral blood and lung tissue were further assessed at 8 weeks to determine the role of cytokines induced by LPG periodontitis, and the effect of P. gingivalis was evaluated using P. gingivalis-IgG and P. gingivalis gingipain. Results Alveolar bone resorption was more severe in the L and LPG groups. However, pulmonary inflammation was observed only in the LPG group at 8 weeks when cytokines and neutrophils in the peripheral blood and lung tissue were the most significant elevation, along with higher levels of P. gingivalis-IgG and P. gingivalis gingipain. Cytokine levels were also increased in the gingival tissue, peripheral blood and lung tissue in the L group, accompanied by elevated peripheral blood neutrophils, but not as significantly as that in the LPG group. Conclusions LPG periodontitis can trigger pulmonary inflammation over the long term, in which cytokines and P. gingivalis play an important role.