TH1 LYMPHOKINE PRODUCTION PROFILES OF NICKEL-SPECIFIC CD4+ LYMPHOCYTE-T CLONES FROM NICKEL CONTACT ALLERGIC AND NONALLERGIC INDIVIDUALS
TH1 LYMPHOKINE PRODUCTION PROFILES OF NICKEL-SPECIFIC CD4+ LYMPHOCYTE-T CLONES FROM NICKEL CONTACT ALLERGIC AND NONALLERGIC INDIVIDUALS
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DOI：
10.1111/1523-1747.ep12494841
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发表时间：
1992-01-01
影响因子：
6.5
通讯作者：
BOS, JD
中科院分区：
文献类型：
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作者：
KAPSENBERG, ML;WIERENGA, EA;BOS, JD
从镍过敏和非过敏供体制备镍特异性T淋巴细胞克隆（TLC）组。 两组的TLC显示TCR-α/β、CD 4、CD 2、CD 25和CD 29的表达水平相似，并识别与HLA-DR、HLA-DP和HLA-DQ中具有限制性决定簇的II类HLA分子相关的镍。 在抗原特异性或非特异性刺激后，在TLC中分析来自两组的淋巴因子分泌，并与预先建立的人atopen-specific Th 1和Th 2细胞的代表物的分泌谱进行比较。 来自两组的镍特异性TLC显示与atopen特异性Th 1细胞相似的淋巴因子分泌模式，尽管克隆与克隆之间存在一些差异。 大多数TLC分泌大量的IFN-γ、IL-2、TNF-α和GM-CSF，但很少或没有IL-4和IL-5。 观察到的变化主要涉及IL-2分泌，在一些TLC中可能较低或不存在。 在不同的刺激模式下，包括通过CD 3、CD 2或CD 28激活，一般分泌模式没有变化。 由于镍特异性TLC从过敏和非过敏的个人表现出类似的Th 1分泌模式，目前的结果没有证据表明，异常的淋巴因子分泌的CD 4 + T细胞决定的接触过敏状态，如在以前的研究中发现的特应性过敏。
Panels of nickel-specific T-lymphocyte clones (TLC) were prepared from nickel-allergic and non-allergic donors. TLC from both panels showed similar levels of expression of TCR-alpha/beta, CD4, CD2, CD25, and CD29 and recognized nickel in association with class II HLA molecules with restriction determinants in HLA-DR, HLA-DP, and HLA-DQ. The lymphokine secretion was analyzed in TLC from both panels upon antigen-specific or non-specific stimulation and was compared with the secretion profiles of representants of pre-established human atopen-specific Th1 and Th2 cells. Nickel-specific TLC from both panels showed a lymphokine secretion pattern similar to the atopen-specific Th1 cells, although there was some variation from clone to clone. Most TLC secreted substantial amounts of IFN-gamma, IL-2, TNF-alpha, and GM-CSF, but little or no IL-4 and IL-5. The variation observed mainly concerned IL-2 secretion that could be low or absent in some of the TLC. The general secretion pattern did not change upon different modes of stimulation, including activation via CD3, CD2, or CD28. Because nickel-specific TLC from allergic and non-allergic individuals show a similar Th1 secretion pattern, the present results give no evidence that aberrant lymphokine secretion by CD4+ T cells determines the contact allergic state, as was found for atopic allergy in a previous study.