The effects of splenic artery ligation in an experimental model of secondary hypersplenism.
The effects of splenic artery ligation in an experimental model of secondary hypersplenism.
复制标题
脾动脉结扎对继发性脾功能亢进实验模型的影响。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
A. Kartal
中科院分区：
文献类型：
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作者：
M. Şahin;S. Tekin;F. Aksoy;H. Vatansev;M. Seker;M. Avunduk;A. Kartal
目标
通过脾静脉结扎诱导大鼠脾功能亢进，并观察脾动脉结扎对该模型的影响。
背景
一些医疗中心采用选择性动脉栓塞和脾动脉结扎（SAL）来治疗继发性脾功能亢进，但这些方法并不常见。
材料和方法
30 只雄性 Saprague-Dawley 大鼠被分配到三组（n = 10）。第一组进行剖腹手术，第二组和第三组进行剖腹手术和脾静脉结扎。第三周末，第一组和第二组进行剖腹手术，第三组进行脾动脉结扎。每周进行红细胞、白细胞和血小板计数。在第六周结束时，处死动物并取出脾脏进行组织病理学检查。
结果
在第二组和第三组中，脾静脉结扎后，红细胞和血小板计数在第二周结束时显着减少（p < 0.01）。在仅接受脾静脉结扎的第二组中，该水平在整个实验过程中保持较低水平。第三组，脾动脉结扎后，红细胞和血小板计数均上升；脾动脉结扎后三周时该水平与对照组的水平相似（p > 0.05）。在整个实验过程中，白细胞计数没有观察到显着变化（p > 0.05）。
结论
脾静脉结扎成功诱导实验性继发性脾功能亢进。这种状态可以通过脾动脉结扎来改善。
OBJECTIVE
To induce hypersplenism in rats by splenic vein ligation and to investigate the effects of splenic artery ligation in this model.
BACKGROUND
Selective arterial embolisation and splenic artery ligation (SAL) are used in the treatment of secondary hypersplenism in some medical centres but these methods are not common.
MATERIALS AND METHODS
Thirty male Saprague-Dawley rats were allocated to three groups (n = 10). The first group underwent laparotomy, the second and third groups underwent laparotomy and splenic vein ligation. At the end of the third week, laparotomy was performed in the first and second groups and splenic artery ligation in the third group. Erythrocyte, leukocyte and platelet counts were performed weekly. At the end of the sixth week, the animals were sacrificed and the spleens were taken for histopathologic examination.
RESULTS
In the second and third groups, after splenic vein ligation, the erythrocyte and platelet counts were significantly reduced at the end of the second week (p < 0.01). In the second group, which underwent splenic vein ligation only, the levels remained low throughout the experiment. In the third group, after splenic artery ligation, there were rises in both erythrocyte and platelet counts; the levels were similar to the levels in the control group at three weeks after splenic artery ligation (p > 0.05). No significant changes were observed in the leukocyte counts throughout the experiment (p > 0.05).
CONCLUSIONS
Splenic vein ligation successfully induces experimental secondary hypersplenism. This state can be ameliorated by splenic artery ligation.